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Entry of Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus into the hospital: prevalence and population structure in Heidelberg, Germany 2015-2018

Staphylococcus aureus is one of the major pathogens causing community—and healthcare-acquired infections. The presence of the virulence factor Panton-Valentine leukocidin (PVL) is associated with recurrent infection and clinical severity and generally regarded as a feature of community associated-me...

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Hauptverfasser: Klein, Sabrina (VerfasserIn) , Hannesen, Julius (VerfasserIn) , Zanger, Philipp (VerfasserIn) , Heeg, Klaus (VerfasserIn) , Boutin, Sébastien (VerfasserIn) , Nurjadi, Dennis (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 06 August 2020
In: Scientific reports
Year: 2020, Jahrgang: 10
ISSN:2045-2322
DOI:10.1038/s41598-020-70112-z
Online-Zugang:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41598-020-70112-z
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41598-020-70112-z
Volltext
Verfasserangaben:Sabrina Klein, Julius Hannesen, Philipp Zanger, Klaus Heeg, Sébastien Boutin & Dennis Nurjadi
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Zusammenfassung:Staphylococcus aureus is one of the major pathogens causing community—and healthcare-acquired infections. The presence of the virulence factor Panton-Valentine leukocidin (PVL) is associated with recurrent infection and clinical severity and generally regarded as a feature of community associated-methicillin-resistant Staphylococcus aureus (MRSA). To date, the focus of PVL-positive MRSA in hospitalized patients has been on outbreaks. We aimed to investigate whether PVL-positive MRSA has penetrated the community-hospital barrier by determining the prevalence of PVL in MRSA of hospitalized patients. MRSA strains isolated from patients hospitalized > 48 h in Heidelberg University Hospital between 2015 and 2018 Isolates were analysed for the presence of PVL and subjected to spa-typing. PVL-positive MRSA were then characterized by whole genome sequencing. We analysed 740 MRSA isolates in the study period and identified 6.2% (n = 46) PVL-positivity. 32.6% of PVL-positive MRSA met the criteria for nosocomial acquisition. The most frequent clones among the PVL-positive strains were ST80-t044 (21.7%, n = 10/46) and ST8-t008 (19.5%, n = 9/46). WGS identified three possible transmission clusters involving seven patients. In conclusion, we found successful epidemic PVL-positive MRSA clones entering the hospital and causing nosocomial infections. Preventive measures and constant surveillance should be maintained to prevent transmissions and clonal outbreaks.
Beschreibung:Gesehen am 03.11.2020
Beschreibung:Online Resource
ISSN:2045-2322
DOI:10.1038/s41598-020-70112-z

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