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Reduced expression of brain-derived neurotrophic factor in mice deficient for pituitary adenylate cyclase activating polypeptide type-I-receptor

In vitro pituitary adenylate cyclase activating polypeptide (PACAP) induces the expression of brain-derived neurotrophic factor (BDNF) via its specific receptor PAC1. Since BDNF has been implicated in learning paradigms and mice lacking functional PAC1 have deficits in hippocampus-dependent associat...

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Main Authors: Zink, Mathias (Author) , Otto, Christiane (Author) , Zörner, Björn (Author) , Zacher, Christiane (Author) , Schütz, Günther (Author) , Henn, Fritz A. (Author) , Gass, Peter (Author)
Format: Article (Journal)
Language:English
Published: 9 April 2004
In: Neuroscience letters
Year: 2004, Volume: 360, Issue: 1, Pages: 106-108
ISSN:1872-7972
DOI:10.1016/j.neulet.2004.01.030
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.neulet.2004.01.030
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0304394004000928
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Author Notes:Mathias Zink, Christiane Otto, Björn Zörner, Christiane Zacher, Günther Schütz, Fritz A. Henn, Peter Gass
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Summary:In vitro pituitary adenylate cyclase activating polypeptide (PACAP) induces the expression of brain-derived neurotrophic factor (BDNF) via its specific receptor PAC1. Since BDNF has been implicated in learning paradigms and mice lacking functional PAC1 have deficits in hippocampus-dependent associative learning, we investigated whether PAC1 mutants show alterations in hippocampal expression of BDNF and its receptor TrkB. Semi-quantitative in situ-hybridization using exon-specific BDNF-probes revealed significantly reduced expression of the exon-III and exon-V-specific transcripts within the hippocampal CA3 region in PAC1-deficient mice. A similar trend was observed for the exon-I-specific transcript. The expression of the exon-III-specific transcript was also reduced within the dentate gyrus, while Trk B-expression did not differ between genotypes. Our data demonstrate that even in vivo PAC1-mediated signaling seems to play a pivotal role for the transcriptional regulation of BDNF.
Item Description:Gesehen am 03.11.2020
Physical Description:Online Resource
ISSN:1872-7972
DOI:10.1016/j.neulet.2004.01.030

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