Optogenetic control of nuclear protein export
Active nucleocytoplasmic transport is a key mechanism underlying protein regulation in eukaryotes. While nuclear protein import can be controlled in space and time with a portfolio of optogenetic tools, protein export has not been tackled so far. Here we present a light-inducible nuclear export syst...
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| Main Authors: | , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
8 Feb 2016
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| In: |
Nature Communications
Year: 2016, Volume: 7, Pages: 1-9 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/ncomms10624 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/ncomms10624 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/ncomms10624 
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| Author Notes: | Dominik Niopek, Pierre Wehler, Julia Roensch, Roland Eils & Barbara Di Ventura |
| Summary: | Active nucleocytoplasmic transport is a key mechanism underlying protein regulation in eukaryotes. While nuclear protein import can be controlled in space and time with a portfolio of optogenetic tools, protein export has not been tackled so far. Here we present a light-inducible nuclear export system (LEXY) based on a single, genetically encoded tag, which enables precise spatiotemporal control over the export of tagged proteins. A constitutively nuclear, chromatin-anchored LEXY variant expands the method towards light inhibition of endogenous protein export by sequestering cellular CRM1 receptors. We showcase the utility of LEXY for cell biology applications by regulating a synthetic repressor as well as human p53 transcriptional activity with light. LEXY is a powerful addition to the optogenetic toolbox, allowing various novel applications in synthetic and cell biology. |
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| Item Description: | Gesehen am 03.11.2020 |
| Physical Description: | Online Resource |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/ncomms10624 |