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Cell fusion enhances mesendodermal differentiation of human induced pluripotent stem cells

Human induced pluripotent stem cells (iPS cells) resemble embryonic stem cells and can differentiate into cell derivatives of all three germ layers. However, frequently the differentiation efficiency of iPS cells into some lineages is rather poor. Here, we found that fusion of iPS cells with human h...

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Bibliographic Details
Main Authors: Qin, Jie (Author) , Jauch, Anna (Author)
Format: Article (Journal)
Language:English
Published: August 11, 2014
In: Stem Cells and Development
Year: 2014, Volume: 23, Issue: 23, Pages: 2875-2882
ISSN:1557-8534
DOI:10.1089/scd.2014.0120
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1089/scd.2014.0120
Verlag, lizenzpflichtig, Volltext: https://www.liebertpub.com/doi/10.1089/scd.2014.0120
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Author Notes:Jie Qin, Stephanie Sontag, Qiong Lin, Saskia Mitzka, Isabelle Leisten, Rebekka K. Schneider, Xiaoying Wang, Anna Jauch, Michael Peitz, Oliver Brüstle, Wolfgang Wagner, Robert Chunhua Zhao, and Martin Zenke
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Summary:Human induced pluripotent stem cells (iPS cells) resemble embryonic stem cells and can differentiate into cell derivatives of all three germ layers. However, frequently the differentiation efficiency of iPS cells into some lineages is rather poor. Here, we found that fusion of iPS cells with human hematopoietic stem cells (HSCs) enhances iPS cell differentiation. Such iPS hybrids showed a prominent differentiation bias toward hematopoietic lineages but also toward other mesendodermal lineages. Additionally, during differentiation of iPS hybrids, expression of early mesendodermal markers—Brachyury (T), MIX1 Homeobox-Like Protein 1 (MIXL1), and Goosecoid (GSC)—appeared with faster kinetics than in parental iPS cells. Following iPS hybrid differentiation there was a prominent induction of NODAL and inhibition of NODAL signaling blunted mesendodermal differentiation. This indicates that NODAL signaling is critically involved in mesendodermal bias of iPS hybrid differentiation. In summary, we demonstrate that iPS cell fusion with HSCs prominently enhances iPS cell differentiation.
Item Description:Gesehen am 05.11.2020
Physical Description:Online Resource
ISSN:1557-8534
DOI:10.1089/scd.2014.0120

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