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A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells

Coat protein complex I (COPI)-coated vesicles mediate membrane trafficking between Golgi cisternae as well as retrieval of proteins from the Golgi to the endoplasmic reticulum. There are several flavors of the COPI coat defined by paralogous subunits of the protein complex coatomer. However, whether...

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Main Authors: Goyal, Manu Jain (Author) , Zhao, Xiyan (Author) , Bozhinova, Mariya (Author) , Andrade-López, Karla (Author) , Heus, Cecilia de (Author) , Schulze-Dramac, Sandra (Author) , Müller-McNicoll, Michaela (Author) , Klumperman, Judith (Author) , Béthune, Julien (Author)
Format: Article (Journal)
Language:English
Published: 14 July 2020
In: Life science alliance
Year: 2020, Volume: 3, Issue: 9
ISSN:2575-1077
DOI:10.26508/lsa.202000714
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.26508/lsa.202000714
Verlag, lizenzpflichtig, Volltext: https://www.life-science-alliance.org/content/3/9/e202000714
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Author Notes:Manu Jain Goyal, Xiyan Zhao, Mariya Bozhinova, Karla Andrade-López, Cecilia de Heus, Sandra Schulze-Dramac, Michaela Müller-McNicoll, Judith Klumperman, Julien Béthune
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Summary:Coat protein complex I (COPI)-coated vesicles mediate membrane trafficking between Golgi cisternae as well as retrieval of proteins from the Golgi to the endoplasmic reticulum. There are several flavors of the COPI coat defined by paralogous subunits of the protein complex coatomer. However, whether paralogous COPI proteins have specific functions is currently unknown. Here, we show that the paralogous coatomer subunits γ1-COP and γ2-COP are differentially expressed during the neuronal differentiation of mouse pluripotent cells. Moreover, through a combination of genome editing experiments, we demonstrate that whereas γ-COP paralogs are largely functionally redundant, γ1-COP specifically promotes neurite outgrowth. Our work stresses a role of the COPI pathway in neuronal polarization and provides evidence for distinct functions for coatomer paralogous subunits in this process.
Item Description:Gesehen am 09.11.2020
Physical Description:Online Resource
ISSN:2575-1077
DOI:10.26508/lsa.202000714

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