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Alu element-containing RNAs maintain nucleolar structure and function

Abstract Non-coding RNAs play a key role in organizing the nucleus into functional subcompartments. By combining fluorescence microscopy and RNA deep-sequencing-based analysis, we found that RNA polymerase II transcripts originating from intronic Alu elements (aluRNAs) were enriched in the nucleolus...

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Main Authors: Caudron-Herger, Maïwen (Author) , Pankert, Teresa (Author) , Seiler, Jeanette (Author) , Németh, Attila (Author) , Voit, Renate (Author) , Grummt, Ingrid (Author) , Rippe, Karsten (Author)
Format: Article (Journal)
Language:English
Published: 13 October 2015
In: The EMBO journal
Year: 2015, Volume: 34, Issue: 22, Pages: 2758-2774
ISSN:1460-2075
DOI:10.15252/embj.201591458
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.15252/embj.201591458
Verlag, lizenzpflichtig, Volltext: https://www.embopress.org/doi/full/10.15252/embj.201591458
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Author Notes:Maïwen Caudron-Herger, Teresa Pankert, Jeanette Seiler, Attila Németh, Renate Voit, Ingrid Grummt & Karsten Rippe
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Summary:Abstract Non-coding RNAs play a key role in organizing the nucleus into functional subcompartments. By combining fluorescence microscopy and RNA deep-sequencing-based analysis, we found that RNA polymerase II transcripts originating from intronic Alu elements (aluRNAs) were enriched in the nucleolus. Antisense-oligo-mediated depletion of aluRNAs or drug-induced inhibition of RNA polymerase II activity disrupted nucleolar structure and impaired RNA polymerase I-dependent transcription of rRNA genes. In contrast, overexpression of a prototypic aluRNA sequence increased both nucleolus size and levels of pre-rRNA, suggesting a functional link between aluRNA, nucleolus integrity and pre-rRNA synthesis. Furthermore, we show that aluRNAs interact with nucleolin and target ectopic genomic loci to the nucleolus. Our study suggests an aluRNA-based mechanism that links RNA polymerase I and II activities and modulates nucleolar structure and rRNA production.
Item Description:Gesehen am 10.11.2020
Physical Description:Online Resource
ISSN:1460-2075
DOI:10.15252/embj.201591458

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