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Alu element-containing RNAs maintain nucleolar structure and function

Abstract Non-coding RNAs play a key role in organizing the nucleus into functional subcompartments. By combining fluorescence microscopy and RNA deep-sequencing-based analysis, we found that RNA polymerase II transcripts originating from intronic Alu elements (aluRNAs) were enriched in the nucleolus...

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Hauptverfasser: Caudron-Herger, Maïwen (VerfasserIn) , Pankert, Teresa (VerfasserIn) , Seiler, Jeanette (VerfasserIn) , Németh, Attila (VerfasserIn) , Voit, Renate (VerfasserIn) , Grummt, Ingrid (VerfasserIn) , Rippe, Karsten (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 13 October 2015
In: The EMBO journal
Year: 2015, Jahrgang: 34, Heft: 22, Pages: 2758-2774
ISSN:1460-2075
DOI:10.15252/embj.201591458
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.15252/embj.201591458
Verlag, lizenzpflichtig, Volltext: https://www.embopress.org/doi/full/10.15252/embj.201591458
Volltext
Verfasserangaben:Maïwen Caudron-Herger, Teresa Pankert, Jeanette Seiler, Attila Németh, Renate Voit, Ingrid Grummt & Karsten Rippe
Beschreibung
Zusammenfassung:Abstract Non-coding RNAs play a key role in organizing the nucleus into functional subcompartments. By combining fluorescence microscopy and RNA deep-sequencing-based analysis, we found that RNA polymerase II transcripts originating from intronic Alu elements (aluRNAs) were enriched in the nucleolus. Antisense-oligo-mediated depletion of aluRNAs or drug-induced inhibition of RNA polymerase II activity disrupted nucleolar structure and impaired RNA polymerase I-dependent transcription of rRNA genes. In contrast, overexpression of a prototypic aluRNA sequence increased both nucleolus size and levels of pre-rRNA, suggesting a functional link between aluRNA, nucleolus integrity and pre-rRNA synthesis. Furthermore, we show that aluRNAs interact with nucleolin and target ectopic genomic loci to the nucleolus. Our study suggests an aluRNA-based mechanism that links RNA polymerase I and II activities and modulates nucleolar structure and rRNA production.
Beschreibung:Gesehen am 10.11.2020
Beschreibung:Online Resource
ISSN:1460-2075
DOI:10.15252/embj.201591458

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