Combining wide-field super-resolution microscopy and electron tomography: rendering nanoscopic correlative arrays on subcellular architecture
In this chapter, the authors outline in full detail, an uncomplicated approach that enables the combination of wide-field fluorescence super-resolution microscopy with electron tomography, thereby providing an approach that affords the best possible confidence in the structures investigated. The met...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) Chapter/Article |
| Language: | English |
| Published: |
3 October 2014
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| In: |
Correlative light and electron microscopy II
Year: 2014, Pages: 129-149 |
| DOI: | 10.1016/B978-0-12-801075-4.00007-0 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/B9780128010754000070 Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/B978-0-12-801075-4.00007-0 
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| Author Notes: | Filip Braet, Delfine Cheng, Minh Huynh, Jeffrey Henriquez, Gerry Shami, Marko Lampe |
| Summary: | In this chapter, the authors outline in full detail, an uncomplicated approach that enables the combination of wide-field fluorescence super-resolution microscopy with electron tomography, thereby providing an approach that affords the best possible confidence in the structures investigated. The methodical steps to obtain these high-throughput correlative nanoscopic arrays will be visually explored and outlined in detail. The authors will demonstrate the feasibility of the method on cultured Caco-2 colorectal cancer cells that are labeled for filamentous actin. The presented images, morphometric data, and generated models illustrate the strengths of our correlative approach for future advanced structural-biology-oriented questions. Correlative nanoscopy applications can be readily found in which there is a need to reveal biomolecular information at unprecedented resolution on subcellular behavior in various biological and pathobiological processes. |
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| Item Description: | Gesehen am 12.11.2020 |
| Physical Description: | Online Resource |
| DOI: | 10.1016/B978-0-12-801075-4.00007-0 |