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Detecting endogenous SUMO targets in mammalian cells and tissues

SUMOylation is a dynamic protein post-translational modification that regulates many eukaryotic proteins. Now a methodology using commercially available monoclonal antibodies coupled to MS analysis leads to the enrichment and identification of endogenous targets for SUMO1 and for SUMO2/3 in HeLa cel...

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Bibliographic Details
Main Authors: Becker, Janina (Author) , Barysch, Sina-Victoria (Author) , Karaca, Samir (Author) , Dittner, Claudia (Author) , Hsiao, He-Hsuan (Author) , Berriel Diaz, Mauricio (Author) , Herzig, Stephan (Author) , Urlaub, Henning (Author) , Melchior, Frauke (Author)
Format: Article (Journal)
Language:English
Published: 17 March 2013
In: Nature structural & molecular biology
Year: 2013, Volume: 20, Issue: 4, Pages: 525-531
ISSN:1545-9985
DOI:10.1038/nsmb.2526
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/nsmb.2526
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/nsmb.2526
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Author Notes:Janina Becker, Sina V Barysch, Samir Karaca, Claudia Dittner, He-Hsuan Hsiao, Mauricio Berriel Diaz, Stephan Herzig, Henning Urlaub & Frauke Melchior
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Summary:SUMOylation is a dynamic protein post-translational modification that regulates many eukaryotic proteins. Now a methodology using commercially available monoclonal antibodies coupled to MS analysis leads to the enrichment and identification of endogenous targets for SUMO1 and for SUMO2/3 in HeLa cells and mouse liver. This protocol can be adapted for other tissues and organs.
Item Description:Gesehen am 17.11.2020
Physical Description:Online Resource
ISSN:1545-9985
DOI:10.1038/nsmb.2526

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