Detecting endogenous SUMO targets in mammalian cells and tissues

SUMOylation is a dynamic protein post-translational modification that regulates many eukaryotic proteins. Now a methodology using commercially available monoclonal antibodies coupled to MS analysis leads to the enrichment and identification of endogenous targets for SUMO1 and for SUMO2/3 in HeLa cel...

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Hauptverfasser: Becker, Janina (VerfasserIn) , Barysch, Sina-Victoria (VerfasserIn) , Karaca, Samir (VerfasserIn) , Dittner, Claudia (VerfasserIn) , Hsiao, He-Hsuan (VerfasserIn) , Berriel Diaz, Mauricio (VerfasserIn) , Herzig, Stephan (VerfasserIn) , Urlaub, Henning (VerfasserIn) , Melchior, Frauke (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 17 March 2013
In: Nature structural & molecular biology
Year: 2013, Jahrgang: 20, Heft: 4, Pages: 525-531
ISSN:1545-9985
DOI:10.1038/nsmb.2526
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/nsmb.2526
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/nsmb.2526
Volltext
Verfasserangaben:Janina Becker, Sina V Barysch, Samir Karaca, Claudia Dittner, He-Hsuan Hsiao, Mauricio Berriel Diaz, Stephan Herzig, Henning Urlaub & Frauke Melchior
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Zusammenfassung:SUMOylation is a dynamic protein post-translational modification that regulates many eukaryotic proteins. Now a methodology using commercially available monoclonal antibodies coupled to MS analysis leads to the enrichment and identification of endogenous targets for SUMO1 and for SUMO2/3 in HeLa cells and mouse liver. This protocol can be adapted for other tissues and organs.
Beschreibung:Gesehen am 17.11.2020
Beschreibung:Online Resource
ISSN:1545-9985
DOI:10.1038/nsmb.2526