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Alternative splicing of the TNFSF13B (BAFF) pre-mRNA and expression of the BAFFX1 isoform in human immune cells

Human B cell activating factor (TNFSF13B, BAFF) is a tumor necrosis factor superfamily member. Binding its unique receptor (TNFRSF13C, BAFF-R) mediates gene expression and cell survival in B cells via activation of NFκB pathway. Furthermore, there is data indicating a role in T cell function. A func...

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Main Authors: Stelmach, Patrick (Author) , Pütz, Michael (Author) , Pollmann, Robert (Author) , Happel, Michael (Author) , Stei, Susanne (Author) , Schlegel, Kerstin (Author) , Seipelt, Maria (Author) , Eienbröker, Christian (Author) , Eming, Rüdiger (Author) , Mandic, Robert (Author) , Huber, Magdalena (Author) , Tackenberg, Björn (Author)
Format: Article (Journal)
Language:English
Published: 04 August 2020
In: Gene
Year: 2020, Volume: 760
ISSN:1879-0038
DOI:10.1016/j.gene.2020.145021
Online Access:Verlag, Volltext: https://doi.org/10.1016/j.gene.2020.145021
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0378111920306909
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Author Notes:Patrick Stelmach, Michael Pütz, Robert Pollmann, Michael Happel, Susanne Stei, Kerstin Schlegel, Maria Seipelt, Christian Eienbröker, Rüdiger Eming, Robert Mandic, Magdalena Huber, Björn Tackenberg
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Summary:Human B cell activating factor (TNFSF13B, BAFF) is a tumor necrosis factor superfamily member. Binding its unique receptor (TNFRSF13C, BAFF-R) mediates gene expression and cell survival in B cells via activation of NFκB pathway. Furthermore, there is data indicating a role in T cell function. A functionally inhibitory isoform (ΔBAFF) resulting from the deletion of exon 3 in the TNFSF13B pre-RNA has already been reported. However, data on the complexity of post-transcriptional regulation is scarce. Here, we report molecular cloning of nine TNFSF13B transcript variants resulting from alternative splicing of the TNFSF13B pre-mRNA including BAFFX1. This variant is characterized by a partial retention of intron 3 of the TNFSF13B gene causing the appearance of a premature stop codon. We demonstrate the expression of the corresponding BAFFX1 protein in Jurkat T cells, in ex vivo human immune cells and in human tonsillar tissue. Thereby we contribute to the understanding of TNFSF13B gene regulation and reveal that BAFF is regulated through a post-transcriptional mechanism to a greater extent than reported to date.
Item Description:Gesehen am 17.11.2020
Physical Description:Online Resource
ISSN:1879-0038
DOI:10.1016/j.gene.2020.145021

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