Computerized image analysis of iron-stained macrophages
Analysis of iron levels in single cells is critical to understand the consequences of impaired regulation of iron homeostasis. Here we establish a method to analyze intracellular iron deposits by computerized image analysis of Prussian blue-stained alveolar macrophages as a test system. We efficient...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
17 April 2013
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| In: |
Annals of hematology
Year: 2013, Volume: 92, Issue: 9, Pages: 1195-1199 |
| ISSN: | 1432-0584 |
| DOI: | 10.1007/s00277-013-1740-2 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s00277-013-1740-2
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| Author Notes: | Karolina Benesova, Sebastian M. Schaefer, Marcus A. Mall, Martina U. Muckenthaler |
| Summary: | Analysis of iron levels in single cells is critical to understand the consequences of impaired regulation of iron homeostasis. Here we establish a method to analyze intracellular iron deposits by computerized image analysis of Prussian blue-stained alveolar macrophages as a test system. We efficiently detected small differences in macrophage steady-state iron levels in Hfe−/− mice as well as inflammation-induced iron sequestration upon lipopolysaccharide instillation. In conclusion, computerized image analysis of single cells is a robust and reproducible tool suitable for iron measurements in small sample sets with limited cell yield. |
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| Item Description: | Gesehen am 23.11.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1432-0584 |
| DOI: | 10.1007/s00277-013-1740-2 |