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Tissue expression and sero-reactivity of tumor-specific antigens in colorectal cancer

The expression of 14 individual and two groups of tumor antigens was characterized for colorectal carcinoma by RT-PCR using 26 colorectal carcinoma specimens, eight cell lines, six samples of patients with inflammatory bowl diseases, and nine specimens from different locations of an individual patie...

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Main Authors: Gerhardt, Axel (Author) , Usener, Dirk (Author) , Keese, Michael (Author) , Sturm, Jörg (Author) , Schadendorf, Dirk (Author) , Eichmüller, Stefan B. (Author)
Format: Article (Journal)
Language:English
Published: 24 January 2004
In: Cancer letters
Year: 2004, Volume: 208, Issue: 2, Pages: 197-206
ISSN:1872-7980
DOI:10.1016/j.canlet.2003.11.021
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.canlet.2003.11.021
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0304383503008115
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Author Notes:Axel Gerhardt, Dirk Usener, Michael Keese, Jörg Sturm, Dirk Schadendorf, Stefan Eichmüller
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Summary:The expression of 14 individual and two groups of tumor antigens was characterized for colorectal carcinoma by RT-PCR using 26 colorectal carcinoma specimens, eight cell lines, six samples of patients with inflammatory bowl diseases, and nine specimens from different locations of an individual patient with a metastasized rectal carcinoma. The most frequently detected mRNAs were MAGE-A1 (58%), GAGE-3-7 (54%), and cTAGE-5a (31%). At medium frequencies (12-19%) we found cTAGE-1, MAGE-A2, se57-1, RAGE-4, and GAGE-1,2,8, while other tumor antigens were expressed rarely (<9%). 85% of the samples were positive for at least one of the most frequently expressed antigens. Using a secondary SEREX approach and sera of eight colorectal cancer patients we found reactive antibodies against recombinant cTAGE-1 (2 sera), se57-1 (2), truncated GAGE (1), and MAGE-A1 (1). We conclude that certain cancer-germline genes can be detected in colorectal cancer and might therefore be promising targets for immunotherapy.
Item Description:Gesehen am 25.11.2020
Physical Description:Online Resource
ISSN:1872-7980
DOI:10.1016/j.canlet.2003.11.021

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