Cross-linker-mediated regulation of actin network organization controls tissue morphogenesis
Contraction of cortical actomyosin networks driven by myosin activation controls cell shape changes and tissue morphogenesis during animal development. In vitro studies suggest that contractility also depends on the geometrical organization of actin filaments. Here we analyze the function of actomyo...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
June 28 2019
|
| In: |
The journal of cell biology
Year: 2019, Volume: 218, Issue: 8, Pages: 2743-2761 |
| ISSN: | 1540-8140 |
| DOI: | 10.1083/jcb.201811127 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1083/jcb.201811127
|
| Author Notes: | Daniel Krueger, Theresa Quinkler, Simon Arnold Mortensen, Carsten Sachse, and Stefano De Renzis |
| Summary: | Contraction of cortical actomyosin networks driven by myosin activation controls cell shape changes and tissue morphogenesis during animal development. In vitro studies suggest that contractility also depends on the geometrical organization of actin filaments. Here we analyze the function of actomyosin network topology in vivo using optogenetic stimulation of myosin-II in Drosophila embryos. We show that early during cellularization, hexagonally arrayed actomyosin fibers are resilient to myosin-II activation. Actomyosin fibers then acquire a ring-like conformation and become contractile and sensitive to myosin-II. This transition is controlled by Bottleneck, a Drosophila unique protein expressed for only a short time during early cellularization, which we show regulates actin bundling. In addition, it requires two opposing actin cross-linkers, Filamin and Fimbrin. Filamin acts synergistically with Bottleneck to facilitate hexagonal patterning, while Fimbrin controls remodeling of the hexagonal network into contractile rings. Thus, actin cross-linking regulates the spatio-temporal organization of actomyosin contraction in vivo, which is critical for tissue morphogenesis. |
|---|---|
| Item Description: | Gesehen am 08.12.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1540-8140 |
| DOI: | 10.1083/jcb.201811127 |