Buchumschlag

Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis

Distal appendages (DAs) of the mother centriole are essential for the initial steps of ciliogenesis in G1/G0 phase of the cell cycle. DAs are released from centrosomes in mitosis by an undefined mechanism. Here, we show that specific DAs lose their centrosomal localization at the G2/M transition in...

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Hauptverfasser: Viol, Linda (VerfasserIn) , Hata, Shoji (VerfasserIn) , Pastor Peidro, Ana (VerfasserIn) , Neuner, Annett (VerfasserIn) , Murke, Florian (VerfasserIn) , Wuchter, Patrick (VerfasserIn) , Ho, Anthony Dick (VerfasserIn) , Giebel, Bernd (VerfasserIn) , Pereira, Gislene (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: February 14 2020
In: The journal of cell biology
Year: 2020, Jahrgang: 219, Heft: 3
ISSN:1540-8140
DOI:10.1083/jcb.201907136
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1083/jcb.201907136
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Verfasserangaben:Linda Viol, Shoji Hata, Ana Pastor-Peidro, Annett Neuner, Florian Murke, Patrick Wuchter, Anthony D. Ho, Bernd Giebel, and Gislene Pereira
Beschreibung
Zusammenfassung:Distal appendages (DAs) of the mother centriole are essential for the initial steps of ciliogenesis in G1/G0 phase of the cell cycle. DAs are released from centrosomes in mitosis by an undefined mechanism. Here, we show that specific DAs lose their centrosomal localization at the G2/M transition in a manner that relies upon Nek2 kinase activity to ensure low DA levels at mitotic centrosomes. Overexpression of active Nek2A, but not kinase-dead Nek2A, prematurely displaced DAs from the interphase centrosomes of immortalized retina pigment epithelial (RPE1) cells. This dramatic impact was also observed in mammary epithelial cells with constitutively high levels of Nek2. Conversely, Nek2 knockout led to incomplete dissociation of DAs and cilia in mitosis. As a consequence, we observed the presence of a cilia remnant that promoted the asymmetric inheritance of ciliary signaling components and supported cilium reassembly after cell division. Together, our data establish Nek2 as an important kinase that regulates DAs before mitosis.
Beschreibung:Gesehen am 17.12.2020
Beschreibung:Online Resource
ISSN:1540-8140
DOI:10.1083/jcb.201907136

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