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Comparison of methods based on different molecular epidemiological markers for typing of mycobacterium tuberculosis complex strains: interlaboratory study of discriminatory power and reproducibility

<h3>ABSTRACT</h3> <p>In this study, the currently known typing methods for<i>Mycobacterium tuberculosis</i> isolates were evaluated with regard to reproducibility, discrimination, and specificity. Therefore, 90 <i>M. tuberculosis</i> complex strains, origina...

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Main Authors: Kremer, Kristin (Author) , Soolingen, D. van (Author) , Frothingham, R. (Author) , Haas, Walter (Author) , Hermans, P. W. M. (Author) , Martín, C. (Author) , Palittapongarnpim, P. (Author) , Plikaytis, B. B. (Author) , Riley, L. W. (Author) , Yakrus, M. A. (Author) , Musser, J. M. (Author) , Embden, J. D. A. van (Author)
Format: Article (Journal)
Language:English
Published: August 1, 1999
In: Journal of clinical microbiology
Year: 1999, Volume: 37, Issue: 8, Pages: 2607-2618
ISSN:1098-660X
DOI:10.1128/JCM.37.8.2607-2618.1999
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1128/JCM.37.8.2607-2618.1999
Verlag, lizenzpflichtig, Volltext: https://jcm.asm.org/content/37/8/2607
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Author Notes:K. Kremer, D. van Soolingen, R. Frothingham, W.H. Haas, P.W. M. Hermans, C. Martín, P. Palittapongarnpim, B.B. Plikaytis, L.W. Riley, M.A. Yakrus, J.M. Musser, J.D.A. van Embden
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Summary:<h3>ABSTRACT</h3> <p>In this study, the currently known typing methods for<i>Mycobacterium tuberculosis</i> isolates were evaluated with regard to reproducibility, discrimination, and specificity. Therefore, 90 <i>M. tuberculosis</i> complex strains, originating from 38 countries, were tested in five restriction fragment length polymorphism (RFLP) typing methods and in seven PCR-based assays. In all methods, one or more repetitive DNA elements were targeted. The strain typing and the DNA fingerprint analysis were performed in the laboratory most experienced in the respective method. To examine intralaboratory reproducibility, blinded duplicate samples were included. The specificities of the various methods were tested by inclusion of 10 non-<i>M. tuberculosis</i> complex strains. All five RFLP typing methods were highly reproducible. The reliability of the PCR-based methods was highest for the mixed-linker PCR, followed by variable numbers of tandem repeat (VNTR) typing and spoligotyping. In contrast, the double repetitive element PCR (DRE-PCR), IS<i>6110</i> inverse PCR, IS<i>6110</i> ampliprinting, and arbitrarily primed PCR (APPCR) typing were found to be poorly reproducible. The 90 strains were best discriminated by IS<i>6110</i> RFLP typing, yielding 84 different banding patterns, followed by mixed-linker PCR (81 patterns), APPCR (71 patterns), RFLP using the polymorphic GC-rich sequence as a probe (70 patterns), DRE-PCR (63 patterns), spoligotyping (61 patterns), and VNTR typing (56 patterns). We conclude that for epidemiological investigations, strain differentiation by IS<i>6110</i> RFLP or mixed-linker PCR are the methods of choice. A strong association was found between the results of different genetic markers, indicating a clonal population structure of <i>M. tuberculosis</i> strains. Several separate genotype families within the <i>M. tuberculosis</i> complex could be recognized on the basis of the genetic markers used.</p>
Item Description:Gesehen am 21.12.2020
Physical Description:Online Resource
ISSN:1098-660X
DOI:10.1128/JCM.37.8.2607-2618.1999

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