Ca 2+ and Na + dependence of 3-hydroxyglutarate-induced excitotoxicity in primary neuronal cultures from chick embryo telencephalons
Glutaryl-CoA dehydrogenase deficiency (also known as glutaric aciduria type I) is an autosomal, recessively inherited neurometabolic disorder with a distinct neuropathology characterized by acute encephalopathy during a vulnerable period of brain development. Neuronal damage in this disease was demo...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2002
|
| In: |
Pediatric research
Year: 2002, Jahrgang: 52, Heft: 2, Pages: 199-206 |
| ISSN: | 1530-0447 |
| DOI: | 10.1203/00006450-200208000-00011 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1203/00006450-200208000-00011 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/pr2002166 
|
| Verfasserangaben: | Stefan Kölker, Georg Köhr, Barbara Ahlemeyer, Jürgen G. Okun, Verena Pawlak, Friederike Hörster, Ertan Mayatepek, Josef Krieglstein, Georg F. Hoffmann |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1743568908 | ||
| 003 | DE-627 | ||
| 005 | 20230428024246.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201228s2002 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1203/00006450-200208000-00011 |2 doi | |
| 035 | |a (DE-627)1743568908 | ||
| 035 | |a (DE-599)KXP1743568908 | ||
| 035 | |a (OCoLC)1341383919 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Kölker, Stefan |e VerfasserIn |0 (DE-588)1022937758 |0 (DE-627)717335771 |0 (DE-576)366197568 |4 aut | |
| 245 | 1 | 0 | |a Ca 2+ and Na + dependence of 3-hydroxyglutarate-induced excitotoxicity in primary neuronal cultures from chick embryo telencephalons |c Stefan Kölker, Georg Köhr, Barbara Ahlemeyer, Jürgen G. Okun, Verena Pawlak, Friederike Hörster, Ertan Mayatepek, Josef Krieglstein, Georg F. Hoffmann |
| 246 | 3 | 3 | |a Ca2+ and Na+ dependence of 3-hydroxyglutarate-induced excitotoxicity in primary neuronal cultures from chick embryo telencephalons |
| 264 | 1 | |c 2002 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Im Titel sind "2+" und "+" hochgestellt | ||
| 500 | |a Gesehen am 28.12.2020 | ||
| 520 | |a Glutaryl-CoA dehydrogenase deficiency (also known as glutaric aciduria type I) is an autosomal, recessively inherited neurometabolic disorder with a distinct neuropathology characterized by acute encephalopathy during a vulnerable period of brain development. Neuronal damage in this disease was demonstrated to involve N-methyl-d-aspartate (NMDA) receptor-mediated neurotoxicity of the endogenously accumulating metabolite 3-hydroxyglutarate (3-OH-GA). However, it remained unclear whether NMDA receptors are directly or indirectly activated and whether 3-OH-GA disturbs the intracellular Ca2+ homeostasis. Here we report that 3-OH-GA activated recombinant NMDA receptors (e.g. NR1/NR2A) but not recombinant α-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptors (e.g. GluR-A/GluR-B) in HEK293 cells. Fluorescence microscopy using fura-2 as Ca2+ indicator revealed that 3-OH-GA increased intracellular Ca2+ concentrations in the presence of extracellular Ca2+ in cultured chick neurons. Similar to glutamate-induced cell damage, 3-OH-GA neurotoxicity was modulated by extracellular Na+. The large cation N-methyl-d-glucamine, which does not permeate NMDA receptor channels, enhanced 3-OH-GA-induced Ca2+ increase and cell damage. In contrast, 3-OH-GA-induced neurotoxicity was reduced after replacement of Na+ by Li+, which permeates NMDA channels but does not affect the Na+/Ca2+ exchanger in the plasma membrane. Spectrophotometric analysis of respiratory chain complexes I-V in submitochondrial particles from bovine heart revealed only a weak inhibition of 3-OH-GA on complex V at the highest concentration tested (10 mM). In conclusion, the present study revealed that NMDA receptor activation and subsequent disturbance of Ca2+ homeostasis contribute to 3-OH-GA-induced cell damage. | ||
| 700 | 1 | |a Koehr, Georg |e VerfasserIn |0 (DE-588)1037422929 |0 (DE-627)755697855 |0 (DE-576)39144770X |4 aut | |
| 700 | 1 | |a Ahlemeyer, Barbara |e VerfasserIn |0 (DE-588)1223947882 |0 (DE-627)1743515332 |4 aut | |
| 700 | 1 | |a Okun, Jürgen G. |d 1968- |e VerfasserIn |0 (DE-588)121578232 |0 (DE-627)705551415 |0 (DE-576)292781296 |4 aut | |
| 700 | 1 | |a Pawlak, Verena |d 1979- |e VerfasserIn |0 (DE-588)143075608 |0 (DE-627)641874995 |0 (DE-576)334897718 |4 aut | |
| 700 | 1 | |a Hörster, Friederike |e VerfasserIn |0 (DE-588)1070020419 |0 (DE-627)823189155 |0 (DE-576)429699220 |4 aut | |
| 700 | 1 | |a Mayatepek, Ertan |e VerfasserIn |0 (DE-588)13349005X |0 (DE-627)546777678 |0 (DE-576)299882454 |4 aut | |
| 700 | 1 | |a Krieglstein, Josef |d 1938- |e VerfasserIn |0 (DE-588)132737779 |0 (DE-627)52592812X |0 (DE-576)299379213 |4 aut | |
| 700 | 1 | |a Hoffmann, Georg F. |d 1957- |e VerfasserIn |0 (DE-588)115652868 |0 (DE-627)077386116 |0 (DE-576)261230042 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Pediatric research |d London [u.a.] Nature Publishing Group : [Verlag nicht ermittelbar], 1967 |g 52(2002), 2, Seite 199-206 |h Online-Ressource |w (DE-627)32482632X |w (DE-600)2031217-9 |w (DE-576)094477191 |x 1530-0447 |7 nnas |a Ca 2+ and Na + dependence of 3-hydroxyglutarate-induced excitotoxicity in primary neuronal cultures from chick embryo telencephalons |
| 773 | 1 | 8 | |g volume:52 |g year:2002 |g number:2 |g pages:199-206 |g extent:8 |a Ca 2+ and Na + dependence of 3-hydroxyglutarate-induced excitotoxicity in primary neuronal cultures from chick embryo telencephalons |
| 856 | 4 | 0 | |u https://doi.org/10.1203/00006450-200208000-00011 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/pr2002166 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20201228 | ||
| 993 | |a Article | ||
| 994 | |a 2002 | ||
| 998 | |g 115652868 |a Hoffmann, Georg F. |m 115652868:Hoffmann, Georg F. |d 910000 |d 910500 |e 910000PH115652868 |e 910500PH115652868 |k 0/910000/ |k 1/910000/910500/ |p 9 |y j | ||
| 998 | |g 13349005X |a Mayatepek, Ertan |m 13349005X:Mayatepek, Ertan |d 910000 |d 910500 |e 910000PM13349005X |e 910500PM13349005X |k 0/910000/ |k 1/910000/910500/ |p 7 | ||
| 998 | |g 1070020419 |a Hörster, Friederike |m 1070020419:Hörster, Friederike |d 910000 |d 910500 |e 910000PH1070020419 |e 910500PH1070020419 |k 0/910000/ |k 1/910000/910500/ |p 6 | ||
| 998 | |g 121578232 |a Okun, Jürgen G. |m 121578232:Okun, Jürgen G. |d 910000 |d 910500 |e 910000PO121578232 |e 910500PO121578232 |k 0/910000/ |k 1/910000/910500/ |p 4 | ||
| 998 | |g 1037422929 |a Koehr, Georg |m 1037422929:Koehr, Georg |p 2 | ||
| 998 | |g 1022937758 |a Kölker, Stefan |m 1022937758:Kölker, Stefan |d 910000 |d 910500 |e 910000PK1022937758 |e 910500PK1022937758 |k 0/910000/ |k 1/910000/910500/ |p 1 |x j | ||
| 999 | |a KXP-PPN1743568908 |e 3828481256 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"titleAlt":[{"title":"Ca2+ and Na+ dependence of 3-hydroxyglutarate-induced excitotoxicity in primary neuronal cultures from chick embryo telencephalons"}],"recId":"1743568908","person":[{"roleDisplay":"VerfasserIn","family":"Kölker","given":"Stefan","role":"aut","display":"Kölker, Stefan"},{"family":"Koehr","roleDisplay":"VerfasserIn","display":"Koehr, Georg","role":"aut","given":"Georg"},{"family":"Ahlemeyer","roleDisplay":"VerfasserIn","given":"Barbara","role":"aut","display":"Ahlemeyer, Barbara"},{"family":"Okun","roleDisplay":"VerfasserIn","given":"Jürgen G.","display":"Okun, Jürgen G.","role":"aut"},{"family":"Pawlak","roleDisplay":"VerfasserIn","given":"Verena","role":"aut","display":"Pawlak, Verena"},{"given":"Friederike","display":"Hörster, Friederike","role":"aut","roleDisplay":"VerfasserIn","family":"Hörster"},{"family":"Mayatepek","roleDisplay":"VerfasserIn","role":"aut","display":"Mayatepek, Ertan","given":"Ertan"},{"role":"aut","display":"Krieglstein, Josef","given":"Josef","roleDisplay":"VerfasserIn","family":"Krieglstein"},{"roleDisplay":"VerfasserIn","family":"Hoffmann","role":"aut","display":"Hoffmann, Georg F.","given":"Georg F."}],"relHost":[{"pubHistory":["1.1967 -"],"recId":"32482632X","id":{"issn":["1530-0447"],"zdb":["2031217-9"],"eki":["32482632X"]},"title":[{"title_sort":"Pediatric research","title":"Pediatric research"}],"note":["Gesehen am 03.09.2025"],"disp":"Ca 2+ and Na + dependence of 3-hydroxyglutarate-induced excitotoxicity in primary neuronal cultures from chick embryo telencephalonsPediatric research","part":{"text":"52(2002), 2, Seite 199-206","volume":"52","issue":"2","extent":"8","year":"2002","pages":"199-206"},"physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"type":{"bibl":"periodical","media":"Online-Ressource"},"origin":[{"publisher":"[Verlag nicht ermittelbar] ; Lippincott Williams & Wilkins","dateIssuedDisp":"1967-","publisherPlace":"London [u.a.] Nature Publishing Group ; Baltimore, Md.","dateIssuedKey":"1967"}]}],"title":[{"title_sort":"Ca 2+ and Na + dependence of 3-hydroxyglutarate-induced excitotoxicity in primary neuronal cultures from chick embryo telencephalons","title":"Ca 2+ and Na + dependence of 3-hydroxyglutarate-induced excitotoxicity in primary neuronal cultures from chick embryo telencephalons"}],"id":{"doi":["10.1203/00006450-200208000-00011"],"eki":["1743568908"]},"note":["Im Titel sind \"2+\" und \"+\" hochgestellt","Gesehen am 28.12.2020"],"physDesc":[{"extent":"8 S."}],"name":{"displayForm":["Stefan Kölker, Georg Köhr, Barbara Ahlemeyer, Jürgen G. Okun, Verena Pawlak, Friederike Hörster, Ertan Mayatepek, Josef Krieglstein, Georg F. Hoffmann"]},"type":{"bibl":"article-journal","media":"Online-Ressource"},"origin":[{"dateIssuedDisp":"2002","dateIssuedKey":"2002"}],"language":["eng"]} | ||
| SRT | |a KOELKERSTECA2ANDNADE2002 | ||