RNA expression profiling from FACS-isolated cells of the Drosophila intestine
This unit describes a protocol for the isolation of Drosophila intestinal cell populations for the purpose of cell type-specific transcriptome profiling. A method to select a cell type of interest labeled with green or yellow fluorescent protein (GFP, YFP) by making use of the GAL4-UAS bipartite sys...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
13 November 2013
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| In: |
Current protocols in stem cell biology
Year: 2013, Volume: 27, Issue: 1, Pages: 2F.2.1-2F.2.12 |
| ISSN: | 1938-8969 |
| DOI: | https://doi.org/10.1002/9780470151808.sc02f02s27 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/https://doi.org/10.1002/9780470151808.sc02f02s27 Verlag, lizenzpflichtig, Volltext: https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/9780470151808.sc02f02s27 
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| Author Notes: | Devanjali Dutta, Jinyi Xiang, Bruce A. Edgar |
| Summary: | This unit describes a protocol for the isolation of Drosophila intestinal cell populations for the purpose of cell type-specific transcriptome profiling. A method to select a cell type of interest labeled with green or yellow fluorescent protein (GFP, YFP) by making use of the GAL4-UAS bipartite system and fluorescent-activated cell sorting (FACS) is presented. Total RNA is isolated from the sorted cells and linear RNA amplification is used to obtain sufficient amounts of high-quality RNA for analysis by microarray, RT-PCR, or RNA sequencing. This method will be useful for quantitative transcriptome comparison across intestinal cell types under normal and various experimental conditions. Curr. Protoc. Stem Cell Biol. 27:2F.2.1-2F.2.12. © 2013 by John Wiley & Sons, Inc. |
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| Item Description: | Gesehen am 05.01.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1938-8969 |
| DOI: | https://doi.org/10.1002/9780470151808.sc02f02s27 |