Organic phase separation opens up new opportunities to interrogate the RNA-binding proteome
Protein-RNA interactions regulate all aspects of RNA metabolism and are crucial to the function of catalytic ribonucleoproteins. Until recently, the available technologies to capture RNA-bound proteins have been biased toward poly(A) RNA-binding proteins (RBPs) or involve molecular labeling, limitin...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
1 March 2020
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| In: |
Current opinion in chemical biology
Year: 2020, Volume: 54, Pages: 70-75 |
| ISSN: | 1879-0402 |
| DOI: | 10.1016/j.cbpa.2020.01.009 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.cbpa.2020.01.009 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S1367593120300107 
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| Author Notes: | Tom Smith, Eneko Villanueva, Rayner M.L. Queiroz, Charlotte S. Dawson, Mohamed Elzek, Erika C. Urdaneta, Anne E. Willis, Benedikt M. Beckmann, Jeroen Krijgsveld, and Kathryn S. Lilley |
| Summary: | Protein-RNA interactions regulate all aspects of RNA metabolism and are crucial to the function of catalytic ribonucleoproteins. Until recently, the available technologies to capture RNA-bound proteins have been biased toward poly(A) RNA-binding proteins (RBPs) or involve molecular labeling, limiting their application. With the advent of organic-aqueous phase separation-based methods, we now have technologies that efficiently enrich the complete suite of RBPs and enable quantification of RBP dynamics. These flexible approaches to study RBPs and their bound RNA open up new research avenues for systems-level interrogation of protein-RNA interactions. |
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| Item Description: | Gesehen am 11.01.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1879-0402 |
| DOI: | 10.1016/j.cbpa.2020.01.009 |