Influence of dietary fatty acid, vegetable, and vitamin intake on etheno-DNA adducts in white blood cells of healthy female volunteers: a pilot study
<p>Etheno-DNA adducts such as 1,<i>N</i><sup>6</sup>-ethenodeoxyadenosine (εdA) and <i>N</i><sup>2</sup>,3-ethenodeoxycytidine (εdC) are formed as result of oxidative stress and lipid peroxidation via reactive alkenals (J. Nair <i>et al.<...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
November 1, 2001
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| In: |
Cancer epidemiology, biomarkers & prevention
Year: 2001, Volume: 10, Issue: 11, Pages: 1187-1191 |
| ISSN: | 1538-7755 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://cebp.aacrjournals.org/content/10/11/1187
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| Author Notes: | Tina Hagenlocher, Jagadeesan Nair, Nikolaus Becker, Andrea Korfmann, and Helmut Bartsch |
| Summary: | <p>Etheno-DNA adducts such as 1,<i>N</i><sup>6</sup>-ethenodeoxyadenosine (εdA) and <i>N</i><sup>2</sup>,3-ethenodeoxycytidine (εdC) are formed as result of oxidative stress and lipid peroxidation via reactive alkenals (J. Nair <i>et al.</i>, Mutat. Res., <i>424:</i>59-69, 1999). High ϖ-6 polyunsaturated fatty acid intake markedly increased levels of WBCs in female volunteers on a controlled diet (J. Nair <i>et al.</i>, Cancer Epidemiol. Biomark. Prev., <i>6:</i>591-601, 1997). In healthy female volunteers we investigated possible correlations between dietary fatty acid intake (by questionnaire), concentration of linoleic acid (LA) and oleic acid (OA) in serum (<i>n</i> = 34), and etheno-DNA adduct levels in WBC (<i>n</i> = 42). Two groups of samples were selected according to dietary intake >15 g (group A) or <5 g (group B) LA/day. Serum samples were analyzed for free OA and LA by gas chromatography-mass spectroscopy and WBC-DNA for εdA and εdC adducts by immunoaffinity <sup>32</sup>P postlabeling. On a group level, serum LA and OA concentrations were higher in group A than group B, whereas the LA/OA ratios were similar. The mean εdA and εdC levels did not significantly differ in groups A and B, but a third of the individuals had more than twice the mean adduct levels than the rest. Correlation analyses revealed a significant inverse correlation for εdA in WBC-DNA and vegetable or vitamin E consumption. We conclude that etheno-DNA adduct levels are not determined by LA intake alone but might depend on the ratio of ϖ-6 polyunsaturated fatty acid:other fatty acids and of antioxidants consumed in the diet. This pilot study also indicated a protective effect of dietary vitamin E and vegetables against miscoding, lipid peroxidation-induced DNA lesions.</p> |
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| Item Description: | Gesehen am 12.01.2001 |
| Physical Description: | Online Resource |
| ISSN: | 1538-7755 |