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Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis

Methods: Using a cross-sectional study design, plasma and peripheral blood mononuclear cells (PBMC) were collected from patients and control subjects. Protein and mRNA (in PBMC) levels for IL-27 and IL-35 (IL27, EBI3, IL12A subunits) as well as IL-27 receptor (IL6ST and IL27RA subunits) were assesse...

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Bibliographische Detailangaben
1. Verfasser: Ringkowski, Sabine (VerfasserIn)
Weitere Verfasser: Herth, Felix (BerichterstatterIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 1 July 2016
In: Respiratory medicine
Year: 2016, Jahrgang: 117, Pages: 243-253
ISSN:1532-3064
DOI:10.1016/j.rmed.2016.06.021
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.rmed.2016.06.021
Verlag, lizenzpflichtig, Volltext: https://linkinghub.elsevier.com/retrieve/pii/S0954611116301470
Volltext
Verfasserangaben:Sabine Ringkowski, Joshua Loke, Shuying Huang, Hasib Ahmadzai, Felix J.F. Herth, Paul S. Thomas, Cristan Herbert
Beschreibung
Zusammenfassung:Methods: Using a cross-sectional study design, plasma and peripheral blood mononuclear cells (PBMC) were collected from patients and control subjects. Protein and mRNA (in PBMC) levels for IL-27 and IL-35 (IL27, EBI3, IL12A subunits) as well as IL-27 receptor (IL6ST and IL27RA subunits) were assessed spontaneously and following direct (LPS) and indirect (anti-CD3/28 activation beads) macrophage stimulation using RT- PCR, ELISA and flow cytometry. - Results: Following stimulation with LPS, PBMC of patients with sarcoidosis displayed significantly enhanced expression of IL27 and EBI3 mRNA (p ¼ 0.020 and p ¼ 0.037 respectively) compared to PBMCs from healthy controls. There was also significantly enhanced production of IL-27 by PBMC from patients with sarcoidosis compared to healthy controls in response to LPS stimulation (p ¼ 0.027). IL6ST mRNA and IL6ST protein were significantly lower in patients with sarcoidosis (mRNA p ¼ 0.0002; MFI p ¼ 0.0015) whilst IL27RA protein levels were significantly higher in patients with sarcoidosis compared to healthy controls (MFI p < 0.0001). Plasma IL-35 protein levels did not differ between control and sarcoidosis subjects (p ¼ 0.23). - Conclusion: These results suggest there may be exaggerated activation of IL-27 signalling in response to LPS in sarcoidosis.
Beschreibung:Gesehen am 21.01.2021
Beschreibung:Online Resource
ISSN:1532-3064
DOI:10.1016/j.rmed.2016.06.021

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