Segregation versus colocalization: orthogonally functionalized binary micropatterned substrates regulate the molecular distribution in focal adhesions

Orthogonally functionalized binary micropatterned substrates are produced using a novel protocol. The use of adequate peptido-mimetics enables an unprecedented segregation of purified αvβ3 and α5β1 integrins in adjacent microislands and evidences the preference of U2OS cells to colocalize such recep...

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Main Authors: Guasch, Judith (Author) , Conings, Bert (Author) , Neubauer, Stefanie (Author) , Rechenmacher, Florian (Author) , Ende, Karen (Author) , Rolli, Claudio Gavino (Author) , Kappel, Christian (Author) , Schaufler, Viktoria (Author) , Micoulet, Alexandre (Author) , Kessler, Horst (Author) , Boyen, Hans-Gerd (Author) , Cavalcanti-Adam, Elisabetta A. (Author) , Spatz, Joachim P. (Author)
Format: Article (Journal)
Language:English
Published: 15 May 2015
In: Advanced materials
Year: 2015, Volume: 27, Issue: 25, Pages: 3737-3747
ISSN:1521-4095
DOI:https://doi.org/10.1002/adma.201500900
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/https://doi.org/10.1002/adma.201500900
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.201500900
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Author Notes:Judith Guasch, Bert Conings, Stefanie Neubauer, Florian Rechenmacher, Karen Ende, Claudio G. Rolli, Christian Kappel, Viktoria Schaufler, Alexandre Micoulet, Horst Kessler, Hans-Gerd Boyen, Elisabetta Ada Cavalcanti‐Adam and Joachim P. Spatz
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Summary:Orthogonally functionalized binary micropatterned substrates are produced using a novel protocol. The use of adequate peptido-mimetics enables an unprecedented segregation of purified αvβ3 and α5β1 integrins in adjacent microislands and evidences the preference of U2OS cells to colocalize such receptors. Moreover, this tendency can be altered by varying the geometry and composition of the micropatterns.
Item Description:Gesehen am 25.01.2021
Physical Description:Online Resource
ISSN:1521-4095
DOI:https://doi.org/10.1002/adma.201500900