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isoSTED nanoscopy with intrinsic beam alignment

Despite the need for isotropic optical resolution in a growing number of applications, the majority of super-resolution fluorescence microscopy setups still do not attain an axial resolution comparable to that in the lateral dimensions. Three-dimensional (3D) nanoscopy implementations that employ on...

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Bibliographic Details
Main Authors: Curdt, Franziska (Author) , Herr, Simon J. (Author) , Lutz, Tobias (Author) , Schmidt, Roman (Author) , Engelhardt, Johann (Author) , Sahl, Steffen Joachim (Author) , Hell, Stefan (Author)
Format: Article (Journal)
Language:English
Published: 18 Nov 2015
In: Optics express
Year: 2015, Volume: 23, Issue: 24, Pages: 30891-3090313
ISSN:1094-4087
DOI:10.1364/OE.23.030891
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1364/OE.23.030891
Verlag, lizenzpflichtig, Volltext: https://www.osapublishing.org/oe/abstract.cfm?uri=oe-23-24-30891
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Author Notes:Franziska Curdt, Simon J. Herr, Tobias Lutz, Roman Schmidt, Johann Engelhardt, Steffen J. Sahl, and Stefan W. Hell
Description
Summary:Despite the need for isotropic optical resolution in a growing number of applications, the majority of super-resolution fluorescence microscopy setups still do not attain an axial resolution comparable to that in the lateral dimensions. Three-dimensional (3D) nanoscopy implementations that employ only a single objective lens typically feature a trade-off between axial and lateral resolution. 4Pi arrangements, in which the sample is illuminated coherently through two opposing lenses, have proven their potential for rendering the resolution isotropic. However, instrument complexity due to a large number of alignment parameters has so far thwarted the dissemination of this approach. Here, we present a 4Pi-STED setup combination, also called isoSTED nanoscope, where the STED and excitation beams are intrinsically co-aligned. A highly robust and convenient 4Pi cavity allows easy handling without the need for readjustments during imaging experiments.
Item Description:Gesehen am 25.01.2021
Physical Description:Online Resource
ISSN:1094-4087
DOI:10.1364/OE.23.030891

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