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The role of integrin-linked kinase in the molecular architecture of focal adhesions

Skip to Next Section - Integrin-mediated focal adhesions (FAs) are large, multi-protein complexes that link the actin cytoskeleton to the extracellular matrix and take part in adhesion-mediated signaling. These adhesions are highly complex and diverse at the molecular level; thus, assigning particul...

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Main Authors: Elad, Nadav (Author) , Volberg, Tova (Author) , Patla, Israel (Author) , Hirschfeld-Warneken, Vera C. (Author) , Grashoff, Carsten (Author) , Spatz, Joachim P. (Author) , Fässler, Reinhard (Author) , Geiger, Benjamin (Author) , Medalia, Ohad (Author)
Format: Article (Journal)
Language:English
Published: 15 September 2013
In: Journal of cell science
Year: 2013, Volume: 126, Issue: 18, Pages: 4099-4107
ISSN:1477-9137
DOI:10.1242/jcs.120295
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1242/jcs.120295
Verlag, lizenzpflichtig, Volltext: https://jcs.biologists.org/content/126/18/4099
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Author Notes:Nadav Elad, Tova Volberg, Israel Patla, Vera Hirschfeld-Warneken, Carsten Grashoff, Joachim P. Spatz, Reinhard Fässler, Benjamin Geiger and Ohad Medalia
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Summary:Skip to Next Section - Integrin-mediated focal adhesions (FAs) are large, multi-protein complexes that link the actin cytoskeleton to the extracellular matrix and take part in adhesion-mediated signaling. These adhesions are highly complex and diverse at the molecular level; thus, assigning particular structural or signaling functions to specific components is highly challenging. Here, we combined functional, structural and biophysical approaches to assess the role of a major FA component, namely, integrin-linked kinase (ILK), in adhesion formation. We show here that ILK plays a key role in the formation of focal complexes, early forms of integrin adhesions, and confirm its involvement in the assembly of fibronectin-bound fibrillar adhesions. Examination of ILK-null fibroblasts by cryo-electron tomography pointed to major structural changes in their FAs, manifested as disarray of the associated actin filaments and an increase in the packing density of FA-related particles. Interestingly, adhesion of the mutant cells to the substrate required a higher ligand density than in control cells. These data indicate that ILK has a key role in integrin adhesion assembly and sub-structure, and in the regulation of the FA-associated cytoskeleton.
Item Description:Gesehen am 01.02.2021
Physical Description:Online Resource
ISSN:1477-9137
DOI:10.1242/jcs.120295

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