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In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids

Bifunctional lipid technology: Cells convert externally added photoactivatable and “clickable” fatty acids into a variety of bifunctional phospholipids that can be covalently linked to their protein-binding partners by irradiation with UV light. Derivatization of the clickable group with a reporter...

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Bibliographic Details
Main Authors: Haberkant, Per (Author) , Sachsenheimer, Timo (Author) , Brügger, Britta (Author) , Gavin, Anne-Claude (Author) , Schultz, Carsten (Author)
Format: Article (Journal)
Language:English
Published: 28 February 2013
In: Angewandte Chemie. International edition
Year: 2013, Volume: 52, Issue: 14, Pages: 4033-4038
ISSN:1521-3773
DOI:https://doi.org/10.1002/anie.201210178
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/https://doi.org/10.1002/anie.201210178
Verlag, lizenzpflichtig, Volltext: https://www.onlinelibrary.wiley.com/doi/abs/10.1002/anie.201210178
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Author Notes:Per Haberkant, Reinout Raijmakers, Marjolein Wildwater, Timo Sachsenheimer, Britta Brügger, Kenji Maeda, Martin Houweling, Anne-Claude Gavin, Carsten Schultz, Gerrit van Meer, Albert J.R. Heck, and Joost C.M. Holthuis
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Summary:Bifunctional lipid technology: Cells convert externally added photoactivatable and “clickable” fatty acids into a variety of bifunctional phospholipids that can be covalently linked to their protein-binding partners by irradiation with UV light. Derivatization of the clickable group with a reporter molecule makes it possible to identify and image the lipid-bound proteins in situ (see scheme).
Item Description:Gesehen am 03.02.2021
Physical Description:Online Resource
ISSN:1521-3773
DOI:https://doi.org/10.1002/anie.201210178

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