Inhibition of tyrosine phosphatases induces apoptosis independent from the CD95 system
The inhibition of protein tyrosine phosphatases by pervanadate, a potent activator of B- and T-cells through the induction of tyrosine phosphorylation and downstream signaling events in different activation cascades, efficiently induced apoptosis in lymphoid cell lines. Pervanadate-elicited apoptosi...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
29 September 1999
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| In: |
Cell death and differentiation
Year: 1999, Volume: 6, Issue: 9, Pages: 833-841 |
| ISSN: | 1476-5403 |
| DOI: | 10.1038/sj.cdd.4400559 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/sj.cdd.4400559 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/4400559 
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| Author Notes: | Steffen P. Hehner, Thomas G. Hofmann, Wulf Dröge and M. Lienhard Schmitz |
| Summary: | The inhibition of protein tyrosine phosphatases by pervanadate, a potent activator of B- and T-cells through the induction of tyrosine phosphorylation and downstream signaling events in different activation cascades, efficiently induced apoptosis in lymphoid cell lines. Pervanadate-elicited apoptosis could be blocked by the tyrosine kinase inhibitor herbimycin A. This apoptotic process involved the activation of caspases 3, 8 and 9, the induction of mitochondrial permeability transition, the release of cytochrome C and the fragmentation of chromosomal DNA. T-cells lacking the CD95 receptor or caspase-8 and T-cells stably overexpressing a transdominant negative form of the adaptor protein FADD were still susceptible to pervanadate-induced apoptosis, excluding the involvement of the CD95 system or other FADD-dependent death receptors. The apoptotic program initiated by the inhibition of tyrosine phosphatases did not require the presence of the tyrosine kinase p56lck or phosphatase CD45, whereas Bcl-2 overexpression protected T-cells from pervanadate-induced cytochrome C release, caspase-8 cleavage and apoptosis. |
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| Item Description: | Gesehen am 09.02.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1476-5403 |
| DOI: | 10.1038/sj.cdd.4400559 |