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Identification of HLA class I dependent immunogenic peptides from clonotypic TCRβ expressed in cutaneous T-cell lymphoma

The clonotypic T-cell receptor (TCR) is a potential target antigen for specific immunotherapy of cutaneous T-cell lymphoma (CTCL). We identified T-cell epitopes from the rearranged TCR β chain of the malignant T-cell population by the “reverse immunology” approach. Peptide-specific T-cell lines were...

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Main Authors: Zeng, Jinyang (Author) , Nguyen, Xuan-Duc (Author) , Klüter, Harald (Author) , Schönhaber, Hiltrud (Author) , Song, Mingxia (Author) , Schwinn, Nicole (Author) , Schadendorf, Dirk (Author) , Goerdt, Sergij (Author) , Eichmüller, Stefan B. (Author)
Format: Article (Journal)
Language:English
Published: 26 September 2006
In: International journal of cancer
Year: 2006, Volume: 119, Issue: 10, Pages: 2476-2480
ISSN:1097-0215
DOI:https://doi.org/10.1002/ijc.22113
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/https://doi.org/10.1002/ijc.22113
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.22113
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Author Notes:Jinyang Zeng, Jan Müller‐Berghaus, Xuan Duc Nguyen, Harald Klüter, Hiltrud Schönhaber, Mingxia Song, Nicole Schwinn, Dirk Schadendorf, Sergij Goerdt, Stefan Eichmüller and Edgar Dippel
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Summary:The clonotypic T-cell receptor (TCR) is a potential target antigen for specific immunotherapy of cutaneous T-cell lymphoma (CTCL). We identified T-cell epitopes from the rearranged TCR β chain of the malignant T-cell population by the “reverse immunology” approach. Peptide-specific T-cell lines were generated against predicted epitopes and tested for the recognition of tumor cells and cells transfected with the full-length DNA coding for TCRV β chain. Two peptides derived from the clonotypic TCRVβ of a HLA-A2 positive patient could induce peptide-specific T cells from peripheral blood mononuclear cells of healthy donors and the patient as assessed by IFN-γ ELISpot assay. Furthermore, the reactive CTLs efficiently recognized autologous Sézary tumor cells, as well as HLA-A2 positive 293 cells transfected with recombinant plasmid expressing the corresponding TCRVβ29 protein. Similar results were obtained in a HLA-A3+ patient for TCRVβ7-Jβ2.7. In conclusion, our experiments show that the TCR β chain harbors epitopes suitable as targets for specific vaccination which might be a promising approach for the specific immunotherapy of cutaneous T-cell lymphoma patients. © 2006 Wiley-Liss, Inc.
Item Description:Gesehen am 09.02.2021
Physical Description:Online Resource
ISSN:1097-0215
DOI:https://doi.org/10.1002/ijc.22113

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