Buchumschlag

Identification of HLA class I dependent immunogenic peptides from clonotypic TCRβ expressed in cutaneous T-cell lymphoma

The clonotypic T-cell receptor (TCR) is a potential target antigen for specific immunotherapy of cutaneous T-cell lymphoma (CTCL). We identified T-cell epitopes from the rearranged TCR β chain of the malignant T-cell population by the “reverse immunology” approach. Peptide-specific T-cell lines were...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Zeng, Jinyang (VerfasserIn) , Nguyen, Xuan-Duc (VerfasserIn) , Klüter, Harald (VerfasserIn) , Schönhaber, Hiltrud (VerfasserIn) , Song, Mingxia (VerfasserIn) , Schwinn, Nicole (VerfasserIn) , Schadendorf, Dirk (VerfasserIn) , Goerdt, Sergij (VerfasserIn) , Eichmüller, Stefan B. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 26 September 2006
In: International journal of cancer
Year: 2006, Jahrgang: 119, Heft: 10, Pages: 2476-2480
ISSN:1097-0215
DOI:https://doi.org/10.1002/ijc.22113
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/https://doi.org/10.1002/ijc.22113
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.22113
Volltext
Verfasserangaben:Jinyang Zeng, Jan Müller‐Berghaus, Xuan Duc Nguyen, Harald Klüter, Hiltrud Schönhaber, Mingxia Song, Nicole Schwinn, Dirk Schadendorf, Sergij Goerdt, Stefan Eichmüller and Edgar Dippel
Beschreibung
Zusammenfassung:The clonotypic T-cell receptor (TCR) is a potential target antigen for specific immunotherapy of cutaneous T-cell lymphoma (CTCL). We identified T-cell epitopes from the rearranged TCR β chain of the malignant T-cell population by the “reverse immunology” approach. Peptide-specific T-cell lines were generated against predicted epitopes and tested for the recognition of tumor cells and cells transfected with the full-length DNA coding for TCRV β chain. Two peptides derived from the clonotypic TCRVβ of a HLA-A2 positive patient could induce peptide-specific T cells from peripheral blood mononuclear cells of healthy donors and the patient as assessed by IFN-γ ELISpot assay. Furthermore, the reactive CTLs efficiently recognized autologous Sézary tumor cells, as well as HLA-A2 positive 293 cells transfected with recombinant plasmid expressing the corresponding TCRVβ29 protein. Similar results were obtained in a HLA-A3+ patient for TCRVβ7-Jβ2.7. In conclusion, our experiments show that the TCR β chain harbors epitopes suitable as targets for specific vaccination which might be a promising approach for the specific immunotherapy of cutaneous T-cell lymphoma patients. © 2006 Wiley-Liss, Inc.
Beschreibung:Gesehen am 09.02.2021
Beschreibung:Online Resource
ISSN:1097-0215
DOI:https://doi.org/10.1002/ijc.22113

Ähnliche Einträge