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Differential expression of the tumor suppressor A-kinase anchor protein 12 in human diffuse and pilocytic astrocytomas is regulated by promoter methylation

The scaffold protein A-kinase anchor protein 12 (AKAP12) exerts tumor suppressor activity and is downregulated in several tumor entities. We characterized AKAP12 expression and regulation in astrocytomas, including pilocytic and diffusely infiltrating astrocytomas. We examined 194 human gliomas and...

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Main Authors: Goeppert, Benjamin (Author) , Schmidt, Christopher Roman (Author) , Geiselhart, Lea (Author) , Dutruel, Céline (Author) , Capper, David (Author) , Renner, Marcus (Author) , Vogel, Monika Nadja (Author) , Zachskorn, Cornelia (Author) , Zinke, Jenny (Author) , Campos, Benito (Author) , Schmezer, Peter (Author) , Popanda, Odilia (Author) , Wick, Wolfgang (Author) , Weller, Michael (Author) , Meyermann, Richard (Author) , Schittenhelm, Jens Florian (Author) , Harter, Patrick Nikolaus (Author) , Simon, Perikles (Author) , Weichert, Wilko (Author) , Schirmacher, Peter (Author) , Plass, Christoph (Author) , Mittelbronn, Michel Guy André (Author)
Format: Article (Journal)
Language:English
Published: 01 October 2013
In: Journal of neuropathology and experimental neurology
Year: 2013, Volume: 72, Issue: 10, Pages: 933-941
ISSN:1554-6578
DOI:10.1097/NEN.0b013e3182a59a88
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1097/NEN.0b013e3182a59a88
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Author Notes:Benjamin Goeppert, MD, Christopher R. Schmidt, PhD, Lea Geiselhart, PhD, Céline Dutruel, PhD, David Capper, MD, Marcus Renner, PhD, Monika Nadja Vogel, MD, Cornelia Zachskorn, TA, Jenny Zinke, Benito Campos, MD, Peter Schmezer, PhD, Odilia Popanda, PhD, Wolfgang Wick, MD, Michael Weller, MD, Richard Meyermann, MD, Jens Schittenhelm, MD, Patrick Nikolaus Harter, MD, Perikles Simon, MD, PhD, Wilko Weichert, MD, Peter Schirmacher, MD, Christoph Plass, MD, and Michel Mittelbronn, MD
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Summary:The scaffold protein A-kinase anchor protein 12 (AKAP12) exerts tumor suppressor activity and is downregulated in several tumor entities. We characterized AKAP12 expression and regulation in astrocytomas, including pilocytic and diffusely infiltrating astrocytomas. We examined 194 human gliomas and 23 normal brain white matter samples by immunohistochemistry or immunoblotting for AKAP12 expression. We further performed quantitative methylation analysis of the AKAP12 promoter by MassARRAY® of normal brain, World Health Organization (WHO) grade I to IV astrocytomas, and glioma cell lines. Our results show that AKAP12 is expressed in a perivascular distribution in normal CNS, strongly upregulated in tumor cells in pilocytic astrocytomas, and weakly expressed in diffuse astrocytomas of WHO grade II to IV. Methyl-ation analyses revealed specific hypermethylation of AKAP12α promoter in WHO grade II to IV astrocytomas. Restoration experiments using 5-aza-2′-deoxycytidine in primary glioblastoma cells decreased AKAP12α promoter methylation and markedly increased AKAP12α mRNA levels. In summary, we demonstrate that AKAP12 is differentially expressed in human astrocytomas showing high expression in pilocytic but low expression in diffuse astrocytomas of all WHO-grades. Our results further indicate that epigenetic mechanisms are involved in silencing AKAP12 in diffuse astrocytomas; however, a tumor suppressive role of AKAP12 in distinct astrocytoma subtypes remains to be determined.
Item Description:Gesehen am 11.02.2020
Physical Description:Online Resource
ISSN:1554-6578
DOI:10.1097/NEN.0b013e3182a59a88

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