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Inhibition of transcription-regulating properties of nonstructural protein 1 (NS1) of parvovirus minute virus of mice by a dominant-negative mutant form of NS1

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Nonstructural protein 1 (NS1) of minute virus of mice is involved in viral DNA replication, transcriptional regulation and cytotoxic action in the host cell. Viral DNA replication is dependent on the ability of NS1 to form homo-oligomers. To investigate whether oligomerization is required for NS1 tr...

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Main Authors: Deleu, Laurent (Author) , Nüesch, Jürg P. F. (Author) , Rommelaere, Jean (Author)
Format: Article (Journal)
Language:English
Published: 01 August 2001
In: Journal of general virology
Year: 2001, Volume: 82, Issue: 8, Pages: 1929-1934
ISSN:1465-2099
DOI:10.1099/0022-1317-82-8-1929
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1099/0022-1317-82-8-1929
Verlag, lizenzpflichtig, Volltext: https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-82-8-1929
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Author Notes:Laurent Deleu, Aurora Pujol, Jürg P.F. Nüesch and Jean Rommelaere
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Summary:Nonstructural protein 1 (NS1) of minute virus of mice is involved in viral DNA replication, transcriptional regulation and cytotoxic action in the host cell. Viral DNA replication is dependent on the ability of NS1 to form homo-oligomers. To investigate whether oligomerization is required for NS1 transcriptional activities, a functionally impaired mutant derivative of NS1 that was able to interact with the wild-type (wt) protein and inhibit its activity in a dominant-negative manner was designed. This mutant provided evidence that transactivation of the parvoviral P38 promoter and transinhibition of a heterologous promoter by NS1 were both affected by the co-expression of the wt and the dominant-negative mutant form of NS1. These results indicate that additional functions of NS1, involved in promoter regulation, require oligomer formation.,
Item Description:Gesehen am 15.02.2021
Physical Description:Online Resource
ISSN:1465-2099
DOI:10.1099/0022-1317-82-8-1929

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