Resolution of experimental and tick-borne Borrelia burgdorferi infection in mice by passive, but not active immunization using recombinant OspC
Vaccination with outer surface protein A (OspA) of Borrelia burgdorferi prevents subsequent infection and disease in both laboratory animals and humans with high efficacy. OspA-based immunity, however, does not affect established infection due to the loss of OspA expression in the vertebrate host. W...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
1999
|
| In: |
European journal of immunology
Year: 1999, Volume: 29, Issue: 3, Pages: 946-957 |
| ISSN: | 1521-4141 |
| DOI: | 0.1002/(SICI)1521-4141(199903)29:03<946::AID-IMMU946>3.0.CO;2-P |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/0.1002/(SICI)1521-4141(199903)29:03<946::AID-IMMU946>3.0.CO;2-P
|
| Author Notes: | Weimin Zhong, LiseGern, Thomas Stehle, Crisan Museteanu, Michael Kramer, Reinhard Wallich, and Markus M. Simon |
| Summary: | Vaccination with outer surface protein A (OspA) of Borrelia burgdorferi prevents subsequent infection and disease in both laboratory animals and humans with high efficacy. OspA-based immunity, however, does not affect established infection due to the loss of OspA expression in the vertebrate host. We show here that repeated passive transfer of mouse and/or rabbit immune sera to recombinant GST-OspC fusion protein resulted in a dose-dependent resolution (1) of fully established arthritis and carditis as well as infection in needle-challenged C.B-17 SCID and (2) of infection in both experimentally and tick-infected BALB/c mice. Unexpectedly, active immunization of disease-susceptible AKR/N mice with GST-OspC only led to prevention but not resolution of disease and infection, in spite of high serum titers of OspC-specific Ab and the expression of ospC in tissue-derived spirochetes. The data suggest that the efficacy of OspC antibody-mediated immunity depends on the immunological history of the recipient and/or environment-dependent regulation of OspC surface expression by spirochetes in vivo. The results encourage further attempts to develop therapeutic vaccination protocols against Lyme disease. |
|---|---|
| Item Description: | First published: 28 March 2006 Gesehen am 17.02.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1521-4141 |
| DOI: | 0.1002/(SICI)1521-4141(199903)29:03<946::AID-IMMU946>3.0.CO;2-P |