In vivo evaluation of combined CK2 inhibition and irradiation in human WiDr tumours
Background/Aim: Casein kinase 2 (CK2) which sustains multiple pro-survival functions in cellular DNA-damage response, is strictly regulated in normal cells but elevated in cancer. CK2 is considered as a potential therapeutic target, and its inhibition has been associated with radiosensitization in m...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
January 5, 2021
|
| In: |
In vivo
Year: 2021, Volume: 35, Issue: 1, Pages: 111-117 |
| ISSN: | 1791-7549 |
| DOI: | 10.21873/invivo.12238 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.21873/invivo.12238 Verlag, lizenzpflichtig, Volltext: https://iv.iiarjournals.org/content/35/1/111 
|
| Author Notes: | Felix Zwicker, Henrik Hauswald, Klaus-Josef Weber, Jürgen Debus and Peter E. Huber |
| Summary: | Background/Aim: Casein kinase 2 (CK2) which sustains multiple pro-survival functions in cellular DNA-damage response, is strictly regulated in normal cells but elevated in cancer. CK2 is considered as a potential therapeutic target, and its inhibition has been associated with radiosensitization in mammalian cells in vitro. Here, we investigated potential radiosensitization by CK2 inhibition in vivo. Materials and Methods: The effect of CK2 inhibition in vivo was investigated in human WiDr-xenograft tumours grown subcutaneously on BALB/c nu/nu mice with and without fractionated irradiation. CK2 inhibition was performed using the specific inhibitor tetra-bromobenzotriazole (TBB). Histological examinations included staining for apoptosis and double-strand breaks. Results: Both TBB treatment alone and radiation alone significantly reduced tumour growth, which was reflected by increased apoptosis rates. However, TBB treatment did not boost radiation-induced tumour growth suppression in combined treatment, although the apoptosis rate increased and repair of double-strand breaks was reduced. This was in stark contrast to previous data on in vitro radiosensitization. Conclusion: The absence of radiosensitization by CK2 inhibition should be investigated in different tumour models. |
|---|---|
| Item Description: | Gesehen am 23.02.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1791-7549 |
| DOI: | 10.21873/invivo.12238 |