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In vivo evaluation of combined CK2 inhibition and irradiation in human WiDr tumours

Background/Aim: Casein kinase 2 (CK2) which sustains multiple pro-survival functions in cellular DNA-damage response, is strictly regulated in normal cells but elevated in cancer. CK2 is considered as a potential therapeutic target, and its inhibition has been associated with radiosensitization in m...

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Hauptverfasser: Zwicker, Felix (VerfasserIn) , Hauswald, Henrik (VerfasserIn) , Weber, Klaus-Josef (VerfasserIn) , Debus, Jürgen (VerfasserIn) , Huber, Peter E. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: January 5, 2021
In: In vivo
Year: 2021, Jahrgang: 35, Heft: 1, Pages: 111-117
ISSN:1791-7549
DOI:10.21873/invivo.12238
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.21873/invivo.12238
Verlag, lizenzpflichtig, Volltext: https://iv.iiarjournals.org/content/35/1/111
Volltext
Verfasserangaben:Felix Zwicker, Henrik Hauswald, Klaus-Josef Weber, Jürgen Debus and Peter E. Huber
Beschreibung
Zusammenfassung:Background/Aim: Casein kinase 2 (CK2) which sustains multiple pro-survival functions in cellular DNA-damage response, is strictly regulated in normal cells but elevated in cancer. CK2 is considered as a potential therapeutic target, and its inhibition has been associated with radiosensitization in mammalian cells in vitro. Here, we investigated potential radiosensitization by CK2 inhibition in vivo. Materials and Methods: The effect of CK2 inhibition in vivo was investigated in human WiDr-xenograft tumours grown subcutaneously on BALB/c nu/nu mice with and without fractionated irradiation. CK2 inhibition was performed using the specific inhibitor tetra-bromobenzotriazole (TBB). Histological examinations included staining for apoptosis and double-strand breaks. Results: Both TBB treatment alone and radiation alone significantly reduced tumour growth, which was reflected by increased apoptosis rates. However, TBB treatment did not boost radiation-induced tumour growth suppression in combined treatment, although the apoptosis rate increased and repair of double-strand breaks was reduced. This was in stark contrast to previous data on in vitro radiosensitization. Conclusion: The absence of radiosensitization by CK2 inhibition should be investigated in different tumour models.
Beschreibung:Gesehen am 23.02.2021
Beschreibung:Online Resource
ISSN:1791-7549
DOI:10.21873/invivo.12238

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