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Super-resolution RNA imaging using a rhodamine-binding aptamer with fast exchange kinetics

Overcoming limitations of previous fluorescent light-up RNA aptamers for super-resolution imaging, we present RhoBAST, an aptamer that binds a fluorogenic rhodamine dye with fast association and dissociation kinetics. Its intermittent fluorescence emission enables single-molecule localization micros...

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Bibliographic Details
Main Authors: Sünbül, Murat (Author) , Lackner, Jens (Author) , Martin, Annabell (Author) , Englert, Daniel (Author) , Hacene, Benjamin (Author) , Grün, Franziska (Author) , Nienhaus, Karin (Author) , Nienhaus, G. Ulrich (Author) , Jäschke, Andres (Author)
Format: Article (Journal)
Language:English
Published: 11 February 2021
In: Nature biotechnology
Year: 2021, Volume: 39, Pages: 686-690
ISSN:1546-1696
DOI:10.1038/s41587-020-00794-3
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41587-020-00794-3
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41587-020-00794-3
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Author Notes:Murat Sunbul, Jens Lackner, Annabell Martin, Daniel Englert, Benjamin Hacene, Franziska Grün, Karin Nienhaus, G. Ulrich Nienhaus and Andres Jäschke
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Summary:Overcoming limitations of previous fluorescent light-up RNA aptamers for super-resolution imaging, we present RhoBAST, an aptamer that binds a fluorogenic rhodamine dye with fast association and dissociation kinetics. Its intermittent fluorescence emission enables single-molecule localization microscopy with a resolution not limited by photobleaching. We use RhoBAST to image subcellular structures of RNA in live and fixed cells with about 10-nm localization precision and a high signal-to-noise ratio.
Item Description:Das PDF enthält zusätzlich einen Anhang von 8 Seiten
Gesehen am 26.02.2021
Physical Description:Online Resource
ISSN:1546-1696
DOI:10.1038/s41587-020-00794-3

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