Isolation and analysis of components of CD95 (APO-1/Fas) death-inducing signaling complex
CD95 (APO-1/Fas) is an apoptosis-inducing receptor belonging to the tumor necrosis factor receptor superfamily. Multimerization of CD95 leads to instant recruitment of the signaling molecules FADD and caspase-8 to the activated receptor forming the death-inducing signaling complex (DISC). DISC forma...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
25 May 2002
|
| In: |
Methods
Year: 1999, Volume: 17, Issue: 4, Pages: 287-291 |
| ISSN: | 1095-9130 |
| DOI: | 10.1006/meth.1999.0742 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1006/meth.1999.0742 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1046202399907429 
|
| Author Notes: | Carsten Scaffidi, Peter H. Krammer, and Marcus E. Peter |
| Summary: | CD95 (APO-1/Fas) is an apoptosis-inducing receptor belonging to the tumor necrosis factor receptor superfamily. Multimerization of CD95 leads to instant recruitment of the signaling molecules FADD and caspase-8 to the activated receptor forming the death-inducing signaling complex (DISC). DISC formation is the first essential step of CD95 signaling and results in activation of caspase-8 starting a signaling cascade that leads to apoptosis. Here we describe a method for analyzing the CD95 DISC. The method is based on coimmunoprecipitation of the signaling molecules with the activated CD95 receptor followed by Western blot detection of associated molecules. Therefore, this method can analyze the very first signaling events during CD95-mediated apoptosis. |
|---|---|
| Item Description: | Available online 25 May 2002 Elektronische Reproduktion der Druck-Ausgabe Gesehen am 03.03.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1095-9130 |
| DOI: | 10.1006/meth.1999.0742 |