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Extensive 5'-surveillance guards against non-canonical NAD-caps of nuclear mRNAs in yeast

The ubiquitous redox coenzyme nicotinamide adenine dinucleotide (NAD) acts as a non-canonical cap structure on prokaryotic and eukaryotic ribonucleic acids. Here we find that in budding yeast, NAD-RNAs are abundant (>1400 species), short (<170 nt), and mostly correspond to mRNA 5′-ends. The mo...

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Main Authors: Zhang, Yaqing (Author) , Kuster, David (Author) , Schmidt, Tobias Thomas (Author) , Kirrmaier, Daniel (Author) , Nübel, Gabriele (Author) , Ibberson, David (Author) , Benes, Vladimir (Author) , Hombauer, Hans (Author) , Knop, Michael (Author) , Jäschke, Andres (Author)
Format: Article (Journal)
Language:English
Published: 02 November 2020
In: Nature Communications
Year: 2020, Volume: 11, Pages: 1-17
ISSN:2041-1723
DOI:10.1038/s41467-020-19326-3
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-020-19326-3
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-020-19326-3
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Author Notes:Yaqing Zhang, David Kuster, Tobias Schmidt, Daniel Kirrmaier, Gabriele Nübel, David Ibberson, Vladimir Benes, Hans Hombauer, Michael Knop & Andres Jäschke
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Summary:The ubiquitous redox coenzyme nicotinamide adenine dinucleotide (NAD) acts as a non-canonical cap structure on prokaryotic and eukaryotic ribonucleic acids. Here we find that in budding yeast, NAD-RNAs are abundant (>1400 species), short (<170 nt), and mostly correspond to mRNA 5′-ends. The modification percentage of transcripts is low (<5%). NAD incorporation occurs mainly during transcription initiation by RNA polymerase II, which uses distinct promoters with a YAAG core motif for this purpose. Most NAD-RNAs are 3′-truncated. At least three decapping enzymes, Rai1, Dxo1, and Npy1, guard against NAD-RNA at different cellular locations, targeting overlapping transcript populations. NAD-mRNAs are not translatable in vitro. Our work indicates that in budding yeast, most of the NAD incorporation into RNA seems to be disadvantageous to the cell, which has evolved a diverse surveillance machinery to prematurely terminate, decap and reject NAD-RNAs.
Item Description:Gesehen am 09.05.2022
Physical Description:Online Resource
ISSN:2041-1723
DOI:10.1038/s41467-020-19326-3

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