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Evaluation of production protocols for the generation of NY-ESO-1-specific T cells

NY-ESO-1-specific T cells have shown promising activity in the treatment of soft tissue sarcoma (STS). However, standardized protocols for their generation are limited. Particularly, cost-effectiveness considerations of cell production protocols are of importance for conducting clinical studies. In...

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Main Authors: Gong, Wenjie (Author) , Wang, Lei (Author) , Stock, Sophia (Author) , Ni, Ming (Author) , Schubert, Maria-Luisa (Author) , Neuber, Brigitte (Author) , Kleist, Christian (Author) , Hückelhoven-Krauss, Angela (Author) , Wu, Depei (Author) , Müller-Tidow, Carsten (Author) , Schmitt, Anita (Author) , Shiku, Hiroshi (Author) , Schmitt, Michael (Author) , Sellner, Leopold (Author)
Format: Article (Journal)
Language:English
Published: 14 January 2021
In: Cells
Year: 2021, Volume: 10, Issue: 1
ISSN:2073-4409
DOI:10.3390/cells10010152
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/cells10010152
Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/2073-4409/10/1/152
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Author Notes:Wenjie Gong, Lei Wang, Sophia Stock, Ming Ni, Maria-Luisa Schubert, Brigitte Neuber, Christian Kleist, Angela Hückelhoven-Krauss, Depei Wu, Carsten Müller-Tidow, Anita Schmitt, Hiroshi Shiku, Michael Schmitt and Leopold Sellner
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Summary:NY-ESO-1-specific T cells have shown promising activity in the treatment of soft tissue sarcoma (STS). However, standardized protocols for their generation are limited. Particularly, cost-effectiveness considerations of cell production protocols are of importance for conducting clinical studies. In this study, two different NY-ESO-1-specific T cell production protocols were compared. Major differences between protocols 1 and 2 include culture medium, interleukin-2 and retronectin concentrations, T cell activation strategy, and the transduction process. NY-ESO-1-specific T cells generated according to the two protocols were investigated for differences in cell viability, transduction efficiency, T cell expansion, immunophenotype as well as functionality. NY-ESO-1-specific T cells showed similar viability and transduction efficiency between both protocols. Protocol 1 generated higher absolute numbers of NY-ESO-1-specific T cells. However, there was no difference in absolute numbers of NY-ESO-1-specific T cell subsets with less-differentiated phenotypes accounting for efficient in vivo expansion and engraftment. Furthermore, cells generated according to protocol 1 displayed higher capacity of TNF-α generation, but lower cytotoxic capacities. Overall, both protocols provided functional NY-ESO-1-specific T cells. However, compared to protocol 1, protocol 2 is advantageous in terms of cost-effectiveness. Cell production protocols should be designed diligently to achieve a cost-effective cellular product for further clinical evaluation.
Item Description:Gesehen am 04.03.2021
Physical Description:Online Resource
ISSN:2073-4409
DOI:10.3390/cells10010152

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