The role of CAP3 in CD95 signaling: new insights into the mechanism of procaspase-8 activation
Formation of the CD95 (APO-1/Fas) death inducing signaling complex (DISC) plays a central role in CD95 signaling. Previously, CD95 DISC composition was analyzed by two-dimensional gel electrophoresis and four major cytotoxicity-associated proteins (CAP1-4) were found. CAP1 and CAP2 were defined to b...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2006
|
| In: |
Cell death and differentiation
Year: 2005, Volume: 13, Issue: 3, Pages: 489-498 |
| ISSN: | 1476-5403 |
| DOI: | 10.1038/sj.cdd.4401766 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/sj.cdd.4401766 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/4401766 
|
| Author Notes: | A. Golks, D. Brenner, I. Schmitz, C. Watzl, A. Krueger, P.H. Krammer and I.N. Lavrik |
| Summary: | Formation of the CD95 (APO-1/Fas) death inducing signaling complex (DISC) plays a central role in CD95 signaling. Previously, CD95 DISC composition was analyzed by two-dimensional gel electrophoresis and four major cytotoxicity-associated proteins (CAP1-4) were found. CAP1 and CAP2 were defined to be unmodified and phosphorylated FADD, respectively. CAP4 was identified as procaspase-8a. CAP3, however, has remained elusive. In this study, we demonstrate that CAP3 is an intermediate of procaspase-8 processing. CAP3 is generated within seconds of DISC formation and subsequently processed to the prodomain of procaspase-8a that is known as p26 (CAP5). These findings lead to new insights into the mechanism of procaspase-8 processing and apoptosis initiation. |
|---|---|
| Item Description: | Published: 23 September 2005 Gesehen am 04.03.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1476-5403 |
| DOI: | 10.1038/sj.cdd.4401766 |