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Production of antibodies to the constant region of the mouse T-cell-receptor β-chain

To generate antibodies to the constant region of the mouse T-cell-receptor β chain, the corresponding cDNA clone 86T5 was subcloned into the bacterial expression vector pEX2. The induced hybrid protein consisting of a 30 kDa T-cell-receptor segment and a β-galactosidase carrier moiety was used for i...

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Bibliographic Details
Main Authors: Schenkel, Johannes (Author) , Schäfer, Hubert (Author) , Koch, Susanne (Author) , Burger, Reinhard (Author)
Format: Article (Journal)
Language:English
Published: 1991
In: Acta pathologica, microbiologica et immunologica Scandinavica
Year: 1991, Volume: 99, Issue: 1-6, Pages: 99-102
ISSN:1600-0463
DOI:https://doi.org/10.1111/j.1699-0463.1991.tb05126.x
Online Access:Verlag, lizenzpflichtig, Volltext: https://dx.doi.org/https://doi.org/10.1111/j.1699-0463.1991.tb05126.x
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1699-0463.1991.tb05126.x
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Author Notes:Johannes Schenkel, Hubert Schäfer, Susanne Koch, Reinhard Burger
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Summary:To generate antibodies to the constant region of the mouse T-cell-receptor β chain, the corresponding cDNA clone 86T5 was subcloned into the bacterial expression vector pEX2. The induced hybrid protein consisting of a 30 kDa T-cell-receptor segment and a β-galactosidase carrier moiety was used for immunization. In Western blots using lysates of a T-cell-receptor-positive T-cell hybrid, the antisera obtained reacted with a 42 kDa protein under reducing and a 85 kDa protein under nonreducing conditions. The antisera did not react in binding assays with intact T lymphocytes, i.e. membrane-associated T-cell-receptor protein.
Item Description:Gesehen am 05.03.2021
Physical Description:Online Resource
ISSN:1600-0463
DOI:https://doi.org/10.1111/j.1699-0463.1991.tb05126.x

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