Light-dependent and circadian transcription dynamics in vivo recorded with a destabilized luciferase reporter in neurospora
We show that firefly luciferase is a stable protein when expressed at 25°C in Neurospora, which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
December 31, 2013
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| In: |
PLOS ONE
Year: 2013, Volume: 8, Issue: 12, Pages: 1-6 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0083660 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1371/journal.pone.0083660 Verlag, lizenzpflichtig, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0083660 
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| Author Notes: | François Cesbron, Michael Brunner, Axel C.R. Diernfellner |
| Summary: | We show that firefly luciferase is a stable protein when expressed at 25°C in Neurospora, which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription dynamics associated with the Neurospora circadian clock and its blue-light photosensory system over the course of several days. We show that the tool is suitable to faithfully monitor rapid, but also subtle changes in transcription in a medium to high throughput format. |
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| Item Description: | Gesehen am 18.03.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0083660 |