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Light-dependent and circadian transcription dynamics in vivo recorded with a destabilized luciferase reporter in neurospora

We show that firefly luciferase is a stable protein when expressed at 25°C in Neurospora, which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription...

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Hauptverfasser: Cesbron, François (VerfasserIn) , Brunner, Michael (VerfasserIn) , Diernfellner, Axel (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: December 31, 2013
In: PLOS ONE
Year: 2013, Jahrgang: 8, Heft: 12, Pages: 1-6
ISSN:1932-6203
DOI:10.1371/journal.pone.0083660
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1371/journal.pone.0083660
Verlag, lizenzpflichtig, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0083660
Volltext
Verfasserangaben:François Cesbron, Michael Brunner, Axel C.R. Diernfellner

MARC

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650 4 |a Bioluminescence 
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650 4 |a Circadian rhythms 
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650 4 |a Light 
650 4 |a Light pulses 
650 4 |a Luciferase 
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