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Light-dependent and circadian transcription dynamics in vivo recorded with a destabilized luciferase reporter in neurospora

We show that firefly luciferase is a stable protein when expressed at 25°C in Neurospora, which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription...

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Hauptverfasser: Cesbron, François (VerfasserIn) , Brunner, Michael (VerfasserIn) , Diernfellner, Axel (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: December 31, 2013
In: PLOS ONE
Year: 2013, Jahrgang: 8, Heft: 12, Pages: 1-6
ISSN:1932-6203
DOI:10.1371/journal.pone.0083660
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1371/journal.pone.0083660
Verlag, lizenzpflichtig, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0083660
Volltext
Verfasserangaben:François Cesbron, Michael Brunner, Axel C.R. Diernfellner
Beschreibung
Zusammenfassung:We show that firefly luciferase is a stable protein when expressed at 25°C in Neurospora, which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription dynamics associated with the Neurospora circadian clock and its blue-light photosensory system over the course of several days. We show that the tool is suitable to faithfully monitor rapid, but also subtle changes in transcription in a medium to high throughput format.
Beschreibung:Gesehen am 18.03.2021
Beschreibung:Online Resource
ISSN:1932-6203
DOI:10.1371/journal.pone.0083660

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