A novel, diffusely infiltrative xenograft model of human anaplastic oligodendroglioma with mutations in FUBP1, CIC, and IDH1
Oligodendroglioma poses a biological conundrum for malignant adult human gliomas: it is a tumor type that is universally incurable for patients, and yet, only a few of the human tumors have been established as cell populations in vitro or as intracranial xenografts in vivo. Their survival, thus, may...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
March 19, 2013
|
| In: |
PLOS ONE
Year: 2013, Volume: 8, Issue: 3, Pages: 1-11 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0059773 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1371/journal.pone.0059773 Verlag, lizenzpflichtig, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0059773 
|
| Author Notes: | Barbara Klink, Hrvoje Miletic, Daniel Stieber, Peter C. Huszthy, Jaime Alberto Campos Valenzuela, Jörg Balss, Jian Wang, Manja Schubert, Per Øystein Sakariassen, Terje Sundstrøm, Anja Torsvik, Mads Aarhus, Rupavathana Mahesparan, Andreas von Deimling, Lars Kaderali, Simone P. Niclou, Evelin Schröck, Rolf Bjerkvig, Janice M. Nigro |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1751849384 | ||
| 003 | DE-627 | ||
| 005 | 20220819141048.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210319s2013 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.pone.0059773 |2 doi | |
| 035 | |a (DE-627)1751849384 | ||
| 035 | |a (DE-599)KXP1751849384 | ||
| 035 | |a (OCoLC)1341399053 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Klink, Barbara |d 1978- |e VerfasserIn |0 (DE-588)135753716 |0 (DE-627)570587409 |0 (DE-576)300628293 |4 aut | |
| 245 | 1 | 2 | |a A novel, diffusely infiltrative xenograft model of human anaplastic oligodendroglioma with mutations in FUBP1, CIC, and IDH1 |c Barbara Klink, Hrvoje Miletic, Daniel Stieber, Peter C. Huszthy, Jaime Alberto Campos Valenzuela, Jörg Balss, Jian Wang, Manja Schubert, Per Øystein Sakariassen, Terje Sundstrøm, Anja Torsvik, Mads Aarhus, Rupavathana Mahesparan, Andreas von Deimling, Lars Kaderali, Simone P. Niclou, Evelin Schröck, Rolf Bjerkvig, Janice M. Nigro |
| 246 | 3 | 0 | |a one |
| 264 | 1 | |c March 19, 2013 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 08.06.2022 | ||
| 520 | |a Oligodendroglioma poses a biological conundrum for malignant adult human gliomas: it is a tumor type that is universally incurable for patients, and yet, only a few of the human tumors have been established as cell populations in vitro or as intracranial xenografts in vivo. Their survival, thus, may emerge only within a specific environmental context. To determine the fate of human oligodendroglioma in an experimental model, we studied the development of an anaplastic tumor after intracranial implantation into enhanced green fluorescent protein (eGFP) positive NOD/SCID mice. Remarkably after nearly nine months, the tumor not only engrafted, but it also retained classic histological and genetic features of human oligodendroglioma, in particular cells with a clear cytoplasm, showing an infiltrative growth pattern, and harboring mutations of IDH1 (R132H) and of the tumor suppressor genes, FUBP1 and CIC. The xenografts were highly invasive, exhibiting a distinct migration and growth pattern around neurons, especially in the hippocampus, and following white matter tracts of the corpus callosum with tumor cells accumulating around established vasculature. Although tumors exhibited a high growth fraction in vivo, neither cells from the original patient tumor nor the xenograft exhibited significant growth in vitro over a six-month period. This glioma xenograft is the first to display a pure oligodendroglioma histology and expression of R132H. The unexpected property, that the cells fail to grow in vitro even after passage through the mouse, allows us to uniquely investigate the relationship of this oligodendroglioma with the in vivo microenvironment. | ||
| 650 | 4 | |a Cancers and neoplasms | |
| 650 | 4 | |a Hippocampus | |
| 650 | 4 | |a Histology | |
| 650 | 4 | |a Human genetics | |
| 650 | 4 | |a Malignant tumors | |
| 650 | 4 | |a Neurons | |
| 650 | 4 | |a Oligodendroglioma | |
| 650 | 4 | |a Point mutation | |
| 700 | 1 | |a Miletic, Hrvoje |e VerfasserIn |4 aut | |
| 700 | 1 | |a Stieber, Daniel |e VerfasserIn |4 aut | |
| 700 | 1 | |a Huszthy, Peter C. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Valenzuela, Jaime Alberto Campos |e VerfasserIn |4 aut | |
| 700 | 1 | |a Balß, Jörg |d 1976- |e VerfasserIn |0 (DE-588)133843173 |0 (DE-627)557777216 |0 (DE-576)30014296X |4 aut | |
| 700 | 1 | |a Wang, Jian |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schubert, Manja |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sakariassen, Per Øystein |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sundstrøm, Terje |e VerfasserIn |4 aut | |
| 700 | 1 | |a Torsvik, Anja |e VerfasserIn |4 aut | |
| 700 | 1 | |a Aarhus, Mads |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mahesparan, Rupavathana |e VerfasserIn |4 aut | |
| 700 | 1 | |a Deimling, Andreas von |d 1959- |e VerfasserIn |0 (DE-588)103034115X |0 (DE-627)735093946 |0 (DE-576)378138065 |4 aut | |
| 700 | 1 | |a Kaderali, Lars |d 1974- |e VerfasserIn |0 (DE-588)131908898 |0 (DE-627)516072145 |0 (DE-576)298829193 |4 aut | |
| 700 | 1 | |a Niclou, Simone P. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schröck, Evelin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Bjerkvig, Rolf |e VerfasserIn |4 aut | |
| 700 | 1 | |a Nigro, Janice M. |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t PLOS ONE |d San Francisco, California, US : PLOS, 2006 |g 8(2013), 3, Artikel-ID e59773, Seite 1-11 |h Online-Ressource |w (DE-627)523574592 |w (DE-600)2267670-3 |w (DE-576)281331979 |x 1932-6203 |7 nnas |a A novel, diffusely infiltrative xenograft model of human anaplastic oligodendroglioma with mutations in FUBP1, CIC, and IDH1 |
| 773 | 1 | 8 | |g volume:8 |g year:2013 |g number:3 |g elocationid:e59773 |g pages:1-11 |g extent:11 |a A novel, diffusely infiltrative xenograft model of human anaplastic oligodendroglioma with mutations in FUBP1, CIC, and IDH1 |
| 856 | 4 | 0 | |u https://doi.org/10.1371/journal.pone.0059773 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0059773 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210319 | ||
| 993 | |a Article | ||
| 994 | |a 2013 | ||
| 998 | |g 103034115X |a Deimling, Andreas von |m 103034115X:Deimling, Andreas von |d 910000 |d 912000 |e 910000PD103034115X |e 912000PD103034115X |k 0/910000/ |k 1/910000/912000/ |p 14 | ||
| 999 | |a KXP-PPN1751849384 |e 3891068883 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedKey":"2013","dateIssuedDisp":"March 19, 2013"}],"note":["Gesehen am 08.06.2022"],"title":[{"title_sort":"novel, diffusely infiltrative xenograft model of human anaplastic oligodendroglioma with mutations in FUBP1, CIC, and IDH1","title":"A novel, diffusely infiltrative xenograft model of human anaplastic oligodendroglioma with mutations in FUBP1, CIC, and IDH1"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"language":["eng"],"person":[{"family":"Klink","display":"Klink, Barbara","given":"Barbara","role":"aut"},{"display":"Miletic, Hrvoje","family":"Miletic","given":"Hrvoje","role":"aut"},{"role":"aut","given":"Daniel","family":"Stieber","display":"Stieber, Daniel"},{"role":"aut","given":"Peter C.","display":"Huszthy, Peter C.","family":"Huszthy"},{"family":"Valenzuela","display":"Valenzuela, Jaime Alberto Campos","role":"aut","given":"Jaime Alberto Campos"},{"role":"aut","given":"Jörg","family":"Balß","display":"Balß, Jörg"},{"role":"aut","given":"Jian","display":"Wang, Jian","family":"Wang"},{"given":"Manja","role":"aut","display":"Schubert, Manja","family":"Schubert"},{"family":"Sakariassen","display":"Sakariassen, Per Øystein","given":"Per Øystein","role":"aut"},{"display":"Sundstrøm, Terje","family":"Sundstrøm","given":"Terje","role":"aut"},{"role":"aut","given":"Anja","display":"Torsvik, Anja","family":"Torsvik"},{"role":"aut","given":"Mads","display":"Aarhus, Mads","family":"Aarhus"},{"role":"aut","given":"Rupavathana","display":"Mahesparan, Rupavathana","family":"Mahesparan"},{"family":"Deimling","display":"Deimling, Andreas von","role":"aut","given":"Andreas von"},{"given":"Lars","role":"aut","display":"Kaderali, Lars","family":"Kaderali"},{"role":"aut","given":"Simone P.","display":"Niclou, Simone P.","family":"Niclou"},{"role":"aut","given":"Evelin","family":"Schröck","display":"Schröck, Evelin"},{"given":"Rolf","role":"aut","display":"Bjerkvig, Rolf","family":"Bjerkvig"},{"display":"Nigro, Janice M.","family":"Nigro","role":"aut","given":"Janice M."}],"id":{"doi":["10.1371/journal.pone.0059773"],"eki":["1751849384"]},"relHost":[{"id":{"eki":["523574592"],"issn":["1932-6203"],"zdb":["2267670-3"]},"name":{"displayForm":["Public Library of Science"]},"physDesc":[{"extent":"Online-Ressource"}],"pubHistory":["1.2006 -"],"recId":"523574592","type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Schreibweise des Titels bis 2012: PLoS ONE","Gesehen am 20.03.19"],"origin":[{"publisher":"PLOS ; PLoS","dateIssuedKey":"2006","publisherPlace":"San Francisco, California, US ; Lawrence, Kan.","dateIssuedDisp":"2006-"}],"title":[{"title_sort":"PLOS ONE","title":"PLOS ONE"}],"part":{"issue":"3","volume":"8","extent":"11","text":"8(2013), 3, Artikel-ID e59773, Seite 1-11","pages":"1-11","year":"2013"},"language":["eng"],"corporate":[{"role":"isb","display":"Public Library of Science"}],"disp":"A novel, diffusely infiltrative xenograft model of human anaplastic oligodendroglioma with mutations in FUBP1, CIC, and IDH1PLOS ONE"}],"physDesc":[{"extent":"11 S."}],"name":{"displayForm":["Barbara Klink, Hrvoje Miletic, Daniel Stieber, Peter C. Huszthy, Jaime Alberto Campos Valenzuela, Jörg Balss, Jian Wang, Manja Schubert, Per Øystein Sakariassen, Terje Sundstrøm, Anja Torsvik, Mads Aarhus, Rupavathana Mahesparan, Andreas von Deimling, Lars Kaderali, Simone P. Niclou, Evelin Schröck, Rolf Bjerkvig, Janice M. Nigro"]},"recId":"1751849384"} | ||
| SRT | |a KLINKBARBANOVELDIFFU1920 | ||