Cover Image

Quantitative determination of lateral concentration and depth profile of histidine-tagged recombinant proteins probed by grazing incidence X-ray fluorescence

We have demonstrated that the complementary combination of grazing incidence X-ray fluorescence (GIXF) with specular X-ray reflectivity (XRR) can be used to quantitatively determine the density profiles of Ni2+ ions complexed with chelator headgroups as well as S atoms in recombinant proteins anchor...

Full description

Saved in:
Bibliographic Details
Main Authors: Körner, Alexander (Author) , Abuillan, Wasim (Author) , Deichmann, Christina (Author) , Rossetti, Fernanda (Author) , Köhler, Almut (Author) , Konovalov, Oleg V. (Author) , Wedlich, Doris (Author) , Tanaka, Motomu (Author)
Format: Article (Journal)
Language:English
Published: April 15, 2013
In: The journal of physical chemistry. B, Biophysics, biomaterials, liquids, and soft matter
Year: 2013, Volume: 117, Issue: 17, Pages: 5002-5008
ISSN:1520-5207
DOI:10.1021/jp401869t
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/jp401869t
Get full text
Author Notes:Alexander Körner, Wasim Abuillan, Christina Deichmann, Fernanda F. Rossetti, Almut Köhler, Oleg V. Konovalov, Doris Wedlich, and Motomu Tanaka
Description
Summary:We have demonstrated that the complementary combination of grazing incidence X-ray fluorescence (GIXF) with specular X-ray reflectivity (XRR) can be used to quantitatively determine the density profiles of Ni2+ ions complexed with chelator headgroups as well as S atoms in recombinant proteins anchored to lipid monolayers at the air/water interface. First, we prepared phospholipid monolayers incorporating chelator lipid anchors at different molar fractions at the air/water interface. The fine-structures perpendicular to the global plane of monolayers were characterized by XRR in the presence of Ni2+ ions, yielding the thickness, roughness, and electron density of the stratified lipid monolayers. X-ray fluorescence intensities from Ni Kα core levels recorded at the incidence angles below and above the critical angle of total reflection allow for the determination of the position and lateral density of Ni2+ ions associated with chelator headgroups with a high spatial accuracy (±5 Å). The coupling of histidine-tagged Xenopus cadherin 11 (Xcad-11) can also be identified by changes in the fines-structures using XRR. Although fluorescence intensities from S Kα level were much weaker than Ni Kα signals, we could detect the location of S atoms in recombinant Xcad-11 proteins.
Item Description:Gesehen am 08.12.2021
Physical Description:Online Resource
ISSN:1520-5207
DOI:10.1021/jp401869t

Similar Items