An integrated YAC map of the human X chromosome
The human X chromosome is associated with a large number of disease phenotypes, principally because of its unique mode of inheritance that tends to reveal all recessive disorders in males. With the longer term goal of identifying and characterizing most of these genes, we have adopted a chromosome-w...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
1996 Oct
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| In: |
Genome research
Year: 1996, Volume: 6, Issue: 10, Pages: 943-955 |
| ISSN: | 1549-5469 |
| DOI: | 10.1101/gr.6.10.943 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1101/gr.6.10.943 Verlag, lizenzpflichtig, Volltext: https://genome.cshlp.org/content/6/10/943 
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| Author Notes: | Hugues Roest Crollius, Mark T. Ross,Andrei Grigoriev, Catherine J. Knights, Ele Holloway, Joseph Misfud, Kim Li, Martin Playford, Simon G. Gregory, Sean J. Humphray, Alison J. Coffey, Chee Gee See, Sharon Marsh, Radost Vatcheva, Johan Kumlien, Tullio Labella, Veronica Lam, Karl H. Rak, Kieran Todd, Richard Mott, D'vorah Graeser, Gudrun Rappold, Gunther Zehetner, Annemarie Poustka, David R. Bentley, Anthony P. Monaco, and Hans Lehrach |
| Summary: | The human X chromosome is associated with a large number of disease phenotypes, principally because of its unique mode of inheritance that tends to reveal all recessive disorders in males. With the longer term goal of identifying and characterizing most of these genes, we have adopted a chromosome-wide strategy to establish a YAC contig map. We have performed > 3250 inter Alu-PCR product hybridizations to identify overlaps between YAC clones. Positional information associated with many of these YAC clones has been derived from our Reference Library Database and a variety of other public sources. We have constructed a YAC contig map of the X chromosome covering 125 Mb of DNA in 25 contigs and containing 906 YAC clones. These contigs have been verified extensively by FISH and by gel and hybridization fingerprinting techniques. This independently derived map exceeds the coverage of recently reported X chromosome maps built as part of whole-genome YAC maps. |
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| Item Description: | Gesehen am 30.03.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1549-5469 |
| DOI: | 10.1101/gr.6.10.943 |