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Rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA: short communication

Pandemic SARS-CoV-2 infection has rapidly developed into a socioeconomic and humanitarian catastrophe. Basic principles to prevent SARS-CoV-2 transmission are social distancing, face masks, contact tracing and early detection of SARS-CoV-2. To meet these requirements, virtually unlimited test capaci...

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Main Authors: Michel, Detlef (Author) , Danzer, Karin M. (Author) , Groß, Rüdiger (Author) , Conzelmann, Carina (Author) , Müller, Janis A. (Author) , Freischmidt, Axel (Author) , Weishaupt, Jochen H. (Author) , Heller, Sandra (Author) , Münch, Jan (Author) , Michel, Manuela (Author) , Stamminger, Thomas (Author) , Kleger, Alexander (Author) , Otto, Markus (Author)
Format: Article (Journal)
Language:English
Published: 3 September 2020
In: Journal of virological methods
Year: 2020, Volume: 286, Pages: 1-4
ISSN:1879-0984
DOI:10.1016/j.jviromet.2020.113965
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.jviromet.2020.113965
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0166093420302172
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Author Notes:Detlef Michel, Karin M. Danzer, Rüdiger Groß, Carina Conzelmann, Janis A. Müller, Axel Freischmidt, Jochen H. Weishaupt, Sandra Heller, Jan Münch, Manuela Michel, Thomas Stamminger, Alexander Kleger, Markus Otto
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Summary:Pandemic SARS-CoV-2 infection has rapidly developed into a socioeconomic and humanitarian catastrophe. Basic principles to prevent SARS-CoV-2 transmission are social distancing, face masks, contact tracing and early detection of SARS-CoV-2. To meet these requirements, virtually unlimited test capacities delivering results in a rapid and reliable manner are a prerequisite. Here, we provide and validate such a rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA, termed COVID-quick-DET. This straightforward method operates with simple proteinase K treatment and repetitive heating steps with a sensitivity of 94.6% in head-to-head comparisons with kit-based isolation methods. This result is supported by data obtained from serially diluted SARS-CoV-2 virus stocks. Given its cost- and time-effective operation, COVID-quick-DET might be best suited for countries with general shortage or temporary acute scarcity of resources and equipment.
Item Description:Gesehen am 07.04.2021
Physical Description:Online Resource
ISSN:1879-0984
DOI:10.1016/j.jviromet.2020.113965

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