Rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA: short communication
Pandemic SARS-CoV-2 infection has rapidly developed into a socioeconomic and humanitarian catastrophe. Basic principles to prevent SARS-CoV-2 transmission are social distancing, face masks, contact tracing and early detection of SARS-CoV-2. To meet these requirements, virtually unlimited test capaci...
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| Hauptverfasser: | , , , , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
3 September 2020
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| In: |
Journal of virological methods
Year: 2020, Jahrgang: 286, Pages: 1-4 |
| ISSN: | 1879-0984 |
| DOI: | 10.1016/j.jviromet.2020.113965 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.jviromet.2020.113965 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0166093420302172 
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| Verfasserangaben: | Detlef Michel, Karin M. Danzer, Rüdiger Groß, Carina Conzelmann, Janis A. Müller, Axel Freischmidt, Jochen H. Weishaupt, Sandra Heller, Jan Münch, Manuela Michel, Thomas Stamminger, Alexander Kleger, Markus Otto |
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| 520 | |a Pandemic SARS-CoV-2 infection has rapidly developed into a socioeconomic and humanitarian catastrophe. Basic principles to prevent SARS-CoV-2 transmission are social distancing, face masks, contact tracing and early detection of SARS-CoV-2. To meet these requirements, virtually unlimited test capacities delivering results in a rapid and reliable manner are a prerequisite. Here, we provide and validate such a rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA, termed COVID-quick-DET. This straightforward method operates with simple proteinase K treatment and repetitive heating steps with a sensitivity of 94.6% in head-to-head comparisons with kit-based isolation methods. This result is supported by data obtained from serially diluted SARS-CoV-2 virus stocks. Given its cost- and time-effective operation, COVID-quick-DET might be best suited for countries with general shortage or temporary acute scarcity of resources and equipment. | ||
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| 650 | 4 | |a Heating | |
| 650 | 4 | |a Kit-free RNA extraction | |
| 650 | 4 | |a Proteinase K | |
| 650 | 4 | |a SARS-CoV-2 | |
| 700 | 1 | |a Danzer, Karin M. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Groß, Rüdiger |e VerfasserIn |4 aut | |
| 700 | 1 | |a Conzelmann, Carina |e VerfasserIn |4 aut | |
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| 700 | 1 | |a Stamminger, Thomas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kleger, Alexander |e VerfasserIn |4 aut | |
| 700 | 1 | |a Otto, Markus |e VerfasserIn |0 (DE-588)1236714962 |0 (DE-627)1762502267 |4 aut | |
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