Adaptive optics enables three-dimensional single particle tracking at the sub-millisecond scale
We present the integration of an adaptive optics element into a feedback-driven single particle tracking microscope. Our instrument captures three-dimensional (3D) trajectories with down to 130 μs temporal resolution for dynamic studies on the nanoscale. Our 3D beam steering approach tracks particle...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
29 April 2013
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| In: |
Applied physics letters
Year: 2013, Volume: 102, Issue: 17, Pages: 1-4 |
| ISSN: | 1077-3118 |
| DOI: | 10.1063/1.4803538 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1063/1.4803538 Verlag, lizenzpflichtig, Volltext: https://aip.scitation.org/doi/10.1063/1.4803538 
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| Author Notes: | Manuel F. Juette, Felix E. Rivera-Molina, Derek K. Toomre, and Joerg Bewersdorf |
| Summary: | We present the integration of an adaptive optics element into a feedback-driven single particle tracking microscope. Our instrument captures three-dimensional (3D) trajectories with down to 130 μs temporal resolution for dynamic studies on the nanoscale. Our 3D beam steering approach tracks particles over an axial range of >6 μm with ∼2 ms mechanical response times and isolates the sample from any tracking motion. Tracking of transport vesicles containing Alexa488-labeled transferrin glycoprotein in living cells demonstrates the speed and sensitivity of our instrument. |
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| Item Description: | Gesehen am 09.04.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1077-3118 |
| DOI: | 10.1063/1.4803538 |