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An unexpected role for FosB in activation-induced cell death of T cells

The CD95 (APO-1/Fas) system plays a major role in induction of apoptosis in lymphoid and nonlymphoid tissues. The CD95 (APO-1/Fas) ligand (CD95L) is induced in response to a variety of signals including TCR/CD3 stimulation or application of chemotherapeutic drugs. Here we report that an AP-1 site lo...

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Bibliographic Details
Main Authors: Baumann, Sven (Author) , Angel, Peter (Author) , Krammer, Peter H. (Author)
Format: Article (Journal)
Language:English
Published: 5 March 2003
In: Oncogene
Year: 2003, Volume: 22, Issue: 9, Pages: 1333-1339
ISSN:1476-5594
DOI:10.1038/sj.onc.1206126
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/sj.onc.1206126
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/1206126
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Author Notes:Sven Baumann, Jochen Hess, Sören T. Eichhorst, Andreas Krueger, Peter Angel, Peter H. Krammer, and Sabine Kirchhoff
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Summary:The CD95 (APO-1/Fas) system plays a major role in induction of apoptosis in lymphoid and nonlymphoid tissues. The CD95 (APO-1/Fas) ligand (CD95L) is induced in response to a variety of signals including TCR/CD3 stimulation or application of chemotherapeutic drugs. Here we report that an AP-1 site located in the 5′ untranslated region of the CD95L gene is required for TCR/CD3-mediated induction of the human CD95L promoter. Electrophoretic mobility shift assays using nuclear extracts of Jurkat T cells as well as TCR/CD3-restimulated primary human T cells demonstrated specific binding of AP-1, predominantly composed of c-Jun and FosB, to this sequence. Ectopic expression of transdominant negative Jun mutants strongly reduced CD95L promoter activity and activation-induced cell death (AICD), confirming the functional significance of FosB/c-Jun binding. Thus, our results demonstrate an important novel function for FosB dimerized with c-Jun in TCR/CD3-mediated AICD in human T cells.
Item Description:Gesehen am 14.04.2021
Physical Description:Online Resource
ISSN:1476-5594
DOI:10.1038/sj.onc.1206126

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