Roles of CCAAT/enhancer-binding protein in transcriptional regulation of the human IL-5 gene
Abstract Interleukin-5 (IL-5) plays a critical role in the pathogenesis of eosinophil-associated allergic disorders, such as asthma. IL-5 may also play a major role in the development of eosinophilia-associated lymphoproliferative disorders caused by human T lymphotropic virus type?I (HTLV-I). In th...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
29 November 2001
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| In: |
European journal of immunology
Year: 2001, Volume: 31, Issue: 12, Pages: 3694-3703 |
| ISSN: | 1521-4141 |
| DOI: | 10.1002/1521-4141(200112)31:12<3694::AID-IMMU3694>3.0.CO;2-S |
| Online Access: | Verlag, Volltext: https://doi.org/10.1002/1521-4141(200112)31:12<3694::AID-IMMU3694>3.0.CO;2-S Verlag, Volltext: https://onlinelibrary.wiley.com/doi/full/10.1002/1521-4141(200112)31:12%3C3694::AID-IMMU3694%3E3.0.CO;2-S 
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| Author Notes: | Min Li-Weber, Marco Giaisi and Peter H. Krammer |
| Summary: | Abstract Interleukin-5 (IL-5) plays a critical role in the pathogenesis of eosinophil-associated allergic disorders, such as asthma. IL-5 may also play a major role in the development of eosinophilia-associated lymphoproliferative disorders caused by human T lymphotropic virus type?I (HTLV-I). In this study, we have investigated the control mechanisms for IL-5 production and found that ectopic expression of NF-IL6 (C/EBP?) increases endogenous IL-5 mRNA expression. The IL-5 promoter contains four C/EBP consensus sequences. We show here that one of the C/EBP site at ??235 promoter region binds to NF-IL6 protein with high affinity and interacts with NF-IL6 and NF-IL6? (C/EBPδ) in Jurkat T?cells. Mutations within the C/EBP sequence reduced the promoter activity in response to T?cell activation by more than 50?%. In addition, we show that in vivo inducible expression of Tax protein in Jurkat T?cells stably transfected with Tax further increased ionomycin plus phorbol ester stimulated IL-5 promoter activity. The effect of Tax on IL-5 promoter activity was abolished when the C/EBP site was mutated. Thus, the C/EBP site may be also involved in HTLV-I Tax-mediated up-regulation of IL-5 gene expression. Our data suggest that C/EBP proteins may regulate IL-5 gene expression in response to different stimulation signals. |
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| Item Description: | Gesehen am 21.04.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1521-4141 |
| DOI: | 10.1002/1521-4141(200112)31:12<3694::AID-IMMU3694>3.0.CO;2-S |